- Clone
- S19004C (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Killer Cell Lectin Like Receptor C1, KLRC1, NK Cell Receptor A
- Isotype
- Mouse IgG1, κ
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Human peripheral blood lymphocytes were stained with anti-human CD3 (clone UCHT1) PE and anti-human CD159a (NKG2A) (clone S19004C) Brilliant Violet 711™ (left) or mouse IgG1, κ Brilliant Violet 711™ isotype control (right).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 375155 | 25 tests | $221.00 | |||
| 375156 | 100 tests | $435.00 | |||
CD159a, also known as NKG2A or KLRC1, is a type II transmembrane protein. It belongs to the killer cell lectin-like receptor family also known as the NKG2 family. It is expressed on NK and NKT cells and T cell subsets. NKG2A is an inhibitory NK cell receptor and it is expressed as a heterodimer with CD94. In human, NKG2A/CD94 complex interacts HLA-E on target cells and inhibits T cells and NK cell effector functions. Recent studies showed that NKG2A blockade enhances the anti-tumor effect of NK cell and CD8+ T cells.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human NKG2A/CD94-transfectants
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
Clone S19004C does not cross-react with mouse CD159a.
- RRID
-
AB_3106206 (BioLegend Cat. No. 375155)
AB_3106206 (BioLegend Cat. No. 375156)
Antigen Details
- Structure
- Type II transmembrane proteins of the C-type lectin superfamily
- Distribution
-
NK cells, NKT cells, T cell subsets
- Function
- Inhibiting NK cell receptor
- Ligand/Receptor
- HLA-E
- Cell Type
- Lymphocytes, NK cells, NKT cells, T cells
- Biology Area
- Immuno-Oncology, Immunology
- Molecular Family
- Immune Checkpoint Receptors
- Antigen References
-
- Brooks AG, et al. 1999. J Immunol. 162:305-13.
- Kaiser BK, et al. 2008. Proc Natl Acad Sci U S A. 105: 6696-701.
- Braud VM, et al. 2003. Trends Immunol. 24:162-4.
- Kamiya T, et al. 2019. J Clin Invest. 129:2094.
- André P, et al. 2018. Cell. 175:1731.
- Gene ID
- 3821 View all products for this Gene ID
- UniProt
- View information about CD159a on UniProt.org
Other Formats
View All CD159a Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were stained with purifie... -
PE anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were stained with CD3 FIT... -
APC anti-human CD159a (NKG2A) Antibody
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Pacific Blue™ anti-human CD159a (NKG2A) Antibody
Human peripheral blood lymphocytes were stained with CD3 APC... -
PE/Cyanine7 anti-human CD159a (NKG2A) Antibody
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Alexa Fluor® 647 anti-human CD159a (NKG2A) Antibody
Human peripheral blood lymphocytes were stained with CD3 FIT... -
PE/Cyanine5 anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were stained with CD3 FIT... -
APC/Fire™ 750 anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Alexa Fluor® 700 anti-human CD159a (NKG2A) Antibody
Human peripheral blood lymphocytes were stained with CD3 FIT... -
PE/Dazzle™ 594 anti-human CD159a (NKG2A) Antibody
Human peripheral blood lymphocytes were stained with CD3 APC... -
Biotin anti-human CD159a (NKG2A) Antibody
Human peripheral blood lymphocytes were stained with CD3 APC... -
Alexa Fluor® 488 anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP/Cyanine5.5 anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were stained with anti-hu... -
FITC anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were stained with anti-hu... -
TotalSeq™-C1374 anti-human CD159a (NKG2A)
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TotalSeq™-D1374 anti-human CD159a (NKG2A)
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TotalSeq™-A1374 anti-human CD159a (NKG2A)
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APC/Fire™ 810 anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were surface stained with... -
Brilliant Violet 421™ anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were surface stained with... -
Brilliant Violet 605™ anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were surface stained with... -
Brilliant Violet 785™ anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were stained with anti-hu... -
TotalSeq™-B1374 anti-human CD159a (NKG2A)
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PerCP/Fire™ 806 anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP/Fire™ 780 anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Fire™ 810 anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were stained with anti-hu... -
APC/Cyanine7 anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were stained with anti-hu... -
Brilliant Violet 650™ anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Red™ 718 anti-human CD159a (NKG2A) (Flexi-Fluor™)
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Brilliant Violet 711™ anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were stained with anti-hu...