Brilliant Violet 711™ anti-human CD54 Antibody

Pricing & Availability
Clone
HA58 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
ICAM-1, Ly-47
Isotype
Mouse IgG1, κ
HA58_BV-711_CD54_Antibody_100323
Human peripheral blood lymphocytes stained with anti-human CD3 PE and anti-human CD54 (clone HA58) Brilliant Violet 711™ (left) or mouse IgG1, κ Brilliant Violet 711™ isotype control (right).
  • HA58_BV-711_CD54_Antibody_100323
    Human peripheral blood lymphocytes stained with anti-human CD3 PE and anti-human CD54 (clone HA58) Brilliant Violet 711™ (left) or mouse IgG1, κ Brilliant Violet 711™ isotype control (right).
Compare all formats See Brilliant Violet 711™ spectral data
Cat # Size Price Quantity Check Availability
353139 25 tests $180.00
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353140 100 tests $405.00
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Description

CD54 is a 85-110 kD type I transmembrane protein also known as ICAM-1. It is expressed on activated endothelial cells, high endothelial venules, T and B cells, monocytes/macrophages, granulocytes, and dendritic cells. The expression of ICAM-1 can be released from the cell surface. CD54 plays a role in cellular adhesion and is involved in inflammation and leukocyte extravasation. CD54 has also been shown to be the major cellular receptor for rhinovirus. ICAM-1 binds to CD11a/CD18 (LFA-1), CD11b/CD18 (Mac-1), CD11c/CD18 (p150, 95) as well as hyaluronan and fibrinogen.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Colonic cancer BM314 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone HA58 recognizes an epitope located in the extracellular D1 domain of CD543. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.

Application References

(PubMed link indicates BioLegend citation)
  1. Tsujisaki M, et al. 1991. Clin. Exp. Immunol. 85:3.
  2. Kanwar JR, et al. 2003. Cancer Gene Ther. 10:468.
  3. Kohka H, et al. 1998. J. Leukoc. Biol. 64:519.
  4. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
RRID
AB_3097245 (BioLegend Cat. No. 353139)
AB_3097245 (BioLegend Cat. No. 353140)

Antigen Details

Structure
Type I membrane protein, Ig superfamily, 85-110 kD
Distribution

Endothelial cells, T cells and B cells, monocytes/macrophages, granulocytes, and dendritic cells

Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Voraberger G, et al. 1991. J. Immunol. 147:2777.
2. Staunton DE, et al. 1988. Cell 52:925.
3. Greve JM, et al. 1989. Cell 56:839.

Gene ID
3383 View all products for this Gene ID
UniProt
View information about CD54 on UniProt.org
Go To Top Version: 1    Revision Date: 10/03/2023

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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