Brilliant Violet 711™ anti-mouse CD278 (ICOS) Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse ICOS cDNA and ICOS hexahistidine fusion protein

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™ under optimal conditions.

Concentration

0.2 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported applications (for the relevant formats) include: blocking of ligand binding. The LEAF™ format is suggested for blocking studies.

Application References

(PubMed link indicates BioLegend citation)

1. Akbari O, et al. 2002. Nat. Med. 8:1024.
2. Harada H, et al. 2003. J. Clin. Invest. 112:234.
3. McAdam AJ, et al. 2000. J. Immunol. 165:5035. (FC Block)
4. Tan SL, et al. 2006. J. Immunol. 176:2872. PubMed

RRID

AB_3097390 (BioLegend Cat. No. 117431)

Antigen Details

Structure

CD28/CTLA-4, 47-57kD

Distribution

Activated T cells, subset of thymocytes

Function

Costimulates T cell activation, proliferation, humoral immune response

Ligand/Receptor

B7h/B7RP-1/GL-50

Cell Type

T cells, Thymocytes

Biology Area

Costimulatory Molecules, Immunology

Molecular Family

CD Molecules

Antigen References

1. Rudd CE, et al. 2003. Nat. Rev. Immunol. 3:544.
2. McAdam AJ, et al. 2000. J. Immunol. 165:5035.
3. Mak TW, et al. 2003. Nat. Immunol. 4:765

Gene ID

54167 View all products for this Gene ID

UniProt

View information about CD278 on UniProt.org

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Other Formats