PE anti-mouse CD278 (ICOS) Antibody

Pricing & Availability
Clone
7E.17G9 (See other available formats)
Regulatory Status
RUO
Other Names
Inducible COStimulatory molecule, H4
Isotype
Rat IgG2b, κ
7Edot17G9_PE_102809
Con A-stimulated (3 days) C57BL/6 splenocytes stained with 7E.17G9 PE.
  • 7Edot17G9_PE_102809
    Con A-stimulated (3 days) C57BL/6 splenocytes stained with 7E.17G9 PE.
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability
117405 50 µg $117.00
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117406 200 µg $288.00
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Description

The 7E.17G9 antibody reacts with the 47-57 kD ICOS protein, also known as inducible costimulatory molecule, and H4. This protein is homologous to the CD28/CTLA-4 proteins. ICOS is expressed on activated T cells and a subset of thymocytes and can costimulate T cells and induce proliferation. In addition ICOS has been shown to be involved in humoral immune responses (B cell germinal center formation). The ICOS ligand, B7h/B7RP-1 and B7-H2 is constitutively expressed in B cell areas of secondary lymphoid organs and can be induced in other tissues by LPS. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development. ICOS stimulation has been shown to be involved in airway tolerance and the downregulation of pulmonary inflammation.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse ICOS cDNA and ICOS hexahistidine fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of ligand binding. The LEAF™ format is suggested for blocking studies.

Application References

(PubMed link indicates BioLegend citation)
  1. Akbari O, et al. 2002. Nat. Med. 8:1024.
  2. Harada H, et al. 2003. J. Clin. Invest. 112:234.
  3. McAdam AJ, et al. 2000. J. Immunol. 165:5035. (FC Block)
  4. Tan SL, et al. 2006. J. Immunol. 176:2872. PubMed
Product Citations
  1. Bajaj R, et al. 2022. Cell Rep. 40:111429. PubMed
  2. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  3. Miyazaki M et al. 2017. Immunity. 46(5):818-834 . PubMed
  4. Tan X, et al. 2021. Sci Adv. 7: . PubMed
  5. Yu X, et al. 2019. Nat Commun. 10:574. PubMed
  6. Katagiri T, et al. 2019. Mucosal Immunol. 12:p1104. PubMed
  7. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  8. Marcandalli J et al. 2019. Cell. 176(6):1420-1431 . PubMed
  9. Fan MY et al. 2018. Cell reports. 25(5):1204-1213 . PubMed
  10. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  11. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
RRID
AB_961244 (BioLegend Cat. No. 117405)
AB_961244 (BioLegend Cat. No. 117406)

Antigen Details

Structure
CD28/CTLA-4, 47-57kD
Distribution

Activated T cells, subset of thymocytes

Function
Costimulates T cell activation, proliferation, humoral immune response
Ligand/Receptor
B7h/B7RP-1/GL-50
Cell Type
T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Rudd CE, et al. 2003. Nat. Rev. Immunol. 3:544.
2. McAdam AJ, et al. 2000. J. Immunol. 165:5035.
3. Mak TW, et al. 2003. Nat. Immunol. 4:765

Gene ID
54167 View all products for this Gene ID
UniProt
View information about CD278 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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