FITC anti-human CD54 Antibody

Pricing & Availability
Clone
HA58 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
ICAM-1, Ly-47
Isotype
Mouse IgG1, κ
HA58_FITC_082911
Human peripheral blood lymphocytes were stained with CD54 (clone HA58) FITC (filled histogram) or mouse IgG1, κ FITC isotype control (open histogram).
  • HA58_FITC_082911
    Human peripheral blood lymphocytes were stained with CD54 (clone HA58) FITC (filled histogram) or mouse IgG1, κ FITC isotype control (open histogram).
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability
353107 25 tests $89.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
353108 100 tests $218.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD54 is a 85-110 kD type I transmembrane protein also known as ICAM-1. It is expressed on activated endothelial cells, high endothelial venules, T and B cells, monocytes/macrophages, granulocytes, and dendritic cells. The expression of ICAM-1 can be released from the cell surface. CD54 plays a role in cellular adhesion and is involved in inflammation and leukocyte extravasation. CD54 has also been shown to be the major cellular receptor for rhinovirus. ICAM-1 binds to CD11a/CD18 (LFA-1), CD11b/CD18 (Mac-1), CD11c/CD18 (p150, 95) as well as hyaluronan and fibrinogen.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Colonic cancer BM314 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone HA58 recognizes an epitope located in the extracellular D1 domain of CD543. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.

Application References

(PubMed link indicates BioLegend citation)
  1. Tsujisaki M, et al. 1991. Clin. Exp. Immunol. 85:3.
  2. Kanwar JR, et al. 2003. Cancer Gene Ther. 10:468.
  3. Kohka H, et al. 1998. J. Leukoc. Biol. 64:519.
  4. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. George M, et al. 2023. Cells. 12:. PubMed
  2. Kim SH, et al. 2021. Nanomaterials (Basel). 11:. PubMed
  3. Godavarthy PS, et al. 2021. Front Cell Dev Biol. 9:675240. PubMed
  4. Eriksson E, et al. 2016. Gene Ther. 10.1038/gt.2016.80. PubMed
  5. Kho D, et al. 2017. PLoS One. 10.1371/journal.pone.0180267. PubMed
  6. Gao Y, et al. 2017. Stem Cell Res Ther. 10.1186/s13287-017-0548-8. PubMed
  7. Biswas I, et al. 2021. Cell Physiol Biochem. 55:605. PubMed
  8. Van der Meer JM, et al. 2020. Cancer Immunol Immunother. . PubMed
RRID
AB_10900254 (BioLegend Cat. No. 353107)
AB_10900254 (BioLegend Cat. No. 353108)

Antigen Details

Structure
Type I membrane protein, Ig superfamily, 85-110 kD
Distribution

Endothelial cells, T cells and B cells, monocytes/macrophages, granulocytes, and dendritic cells

Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Voraberger G, et al. 1991. J. Immunol. 147:2777.
2. Staunton DE, et al. 1988. Cell 52:925.
3. Greve JM, et al. 1989. Cell 56:839.

Gene ID
3383 View all products for this Gene ID
UniProt
View information about CD54 on UniProt.org
Go To Top Version: 2    Revision Date: 10/01/2014

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account