FITC anti-mouse CD19 Antibody

Pricing & Availability
Clone
1D3/CD19 (See other available formats)
Regulatory Status
RUO
Other Names
B4, CVID3
Isotype
Rat IgG2a, κ
1D3slashCD19_FITC_CD19_Antibody_082516
C57BL/6 mouse splenocytes were stained with CD19 FITC (clone 1D3/CD19, filled histogram) or rat IgG2a, κ FITC isotype control (open histogram).
  • 1D3slashCD19_FITC_CD19_Antibody_082516
    C57BL/6 mouse splenocytes were stained with CD19 FITC (clone 1D3/CD19, filled histogram) or rat IgG2a, κ FITC isotype control (open histogram).
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability
152403 25 µg $71.00
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152404 100 µg $183.00
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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Transfected k562 cells expressing the extracellular domain of murine CD19.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking1,2 and immunoprecipitation1.

Application References

(PubMed link indicates BioLegend citation)
  1. Krop I, et al. 1996. Eur. J. Immunol. 26:238. (FC, Blocking, IP)
  2. Abraham P, et al. 2014. Clin. Exp. Immunol. 175:181. (Blocking)
Product Citations
  1. Peng J, et al. 2023. Am J Reprod Immunol. 89:e13678. PubMed
  2. Cheong LY, et al. 2023. Nat Commun. 14:1213. PubMed
  3. Zhou R, et al. 2022. EBioMedicine. 75:103762. PubMed
  4. Hilfenhaus G, et al. 2021. Cancer Res. 81:619. PubMed
  5. Corral D, et al. 2022. Cell Rep. 39:110715. PubMed
  6. Wu Q, et al. 2023. Cell Rep. 42:112057. PubMed
  7. Gon Y, et al. 2023. PLoS One. 18:e0279389. PubMed
  8. Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed
  9. Chei S, et al. 2020. Front Nutr. 7:583186. PubMed
  10. Miller CM, et al. 2020. J Virol. 94:00:00. PubMed
  11. Hu HJ, et al. 2020. Cell Death Dis. 1.168055556. PubMed
  12. Zhao J, et al. 2019. Nat Commun. 10:899. PubMed
  13. Suzuki Y, et al. 2022. Mol Ther Nucleic Acids. 30:226. PubMed
  14. Liang Y, et al. 2020. J Immunol. 204:2984. PubMed
  15. Davis FM, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2353. PubMed
  16. Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
  17. Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed
  18. Ostendorf BN, et al. 2020. Nat Med. 26:1048. PubMed
  19. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  20. Cha SE, et al. 2021. Oncoimmunology. 10:1899469. PubMed
  21. Deng M, et al. 2020. Nat Commun. 11:2193. PubMed
  22. Pardy RD, et al. 2021. Nat Commun. 12:4051. PubMed
  23. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  24. Tavazoie MF, et al. 2018. Cell. 172:825. PubMed
  25. Zhou G, et al. 2019. FASEB J. 33:11396. PubMed
  26. Subramanian K, et al. 2019. Nat Microbiol. 4:62. PubMed
  27. Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed
  28. Dong X, et al. 2022. Front Immunol. 13:896472. PubMed
  29. Niderla-Bielinska J, et al. 2021. Int J Mol Sci. :22. PubMed
  30. Gomzikova MO, et al. 2020. Pharmaceutics. 12:00. PubMed
  31. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  32. Tanegashima K et al. 2017. EBioMedicine. 24:247-256 . PubMed
  33. Whiteley AM et al. 2017. eLife. 6 pii: e26435. PubMed
  34. Jiang W, et al. 2021. Cell Rep. 37:110112. PubMed
RRID
AB_2629812 (BioLegend Cat. No. 152403)
AB_2629812 (BioLegend Cat. No. 152404)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD.
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells.

Function
Modulates B cell activation and differentiation.
Ligand/Receptor
CD21, CD81, Leu-13.
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org
Go To Top Version: 1    Revision Date: 08/26/2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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