FITC anti-mouse CD80 Antibody

Pricing & Availability
Clone
16-10A1 (See other available formats)
Regulatory Status
RUO
Other Names
B7-1, B7, Ly-53
Isotype
Armenian Hamster IgG
16-10A1
LPS-stimulated (3 days) BALB/c splenocytes stained with 16-10A1 FITC
  • 16-10A1
    LPS-stimulated (3 days) BALB/c splenocytes stained with 16-10A1 FITC
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability
104705 50 µg $71.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
104706 500 µg $204.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Reported Reactivity
Dog
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
CHO cell line transfected with mouse B7 (CD80)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2, in vitro and in vivo blocking of CTLA-4 Ig to CD80 by blocking costimulation of T cells by activated B cells2-4, and immunohistochemical staining of acetone-fixed frozen sections1,4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104747-104752).

Application References

(PubMed link indicates BioLegend citation)
  1. Harlan DM, et al. 1994. P. Natl. Acad. Sci. USA 91:3137. (IHC)
  2. Razi-Wolf Z, et al. 1992. P. Natl. Acad. Sci. USA 89:4210. (Block, IP)
  3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
  4. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
  5. Ma XT, et al. 2006. Cancer Res. 66:1169.
  6. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC)
  7. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  8. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
  9. Misra RS, et al. 2010. J. Exp Med. 207:1775. PubMed
  10. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  11. Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
Product Citations
  1. Akhtar MN, et al. 2022. MBio. 13:e0330921. PubMed
  2. Zhuang WR, et al. 2023. Nat Commun. 14:1675. PubMed
  3. Do-Thi VA, et al. 2023. Cancer Res Commun. 3:80. PubMed
  4. Fu S, et al. 2023. Nat Commun. 14:2248. PubMed
  5. Brigo N, et al. 2023. Cells. 12:. PubMed
  6. Bhaskar A, et al. 2023. iScience. 26:106644. PubMed
  7. Wang D, et al. 2023. Nat Commun. 14:2943. PubMed
  8. Rosina M, et al. 2022. Cell Metab. 34:533. PubMed
  9. Kawai H, et al. 2021. Asian Pac J Allergy Immunol. :. PubMed
  10. Chen C, et al. 2022. Sci Adv. 8:eabo4363. PubMed
  11. Chen Y, et al. 2023. Adv Healthc Mater. 12:e2202085. PubMed
  12. Wang H, et al. 2022. J Nanobiotechnology. 20:513. PubMed
  13. Kimura Y, et al. 2023. Front Immunol. 14:1070196. PubMed
  14. Vesin B, et al. 2022. Mol Ther. 30:2984. PubMed
  15. Kis-Toth K, et al. 2011. J Immunol. 187:1273. PubMed
  16. Jiang Q, et al. 2022. Theranostics. 12:59. PubMed
  17. Gravano D, et al. 2011. PLoS One. 6:e13517. PubMed
  18. Chen L, et al. 2022. Mol Ther Oncolytics. 24:522. PubMed
  19. Xia S, et al. 2014. J Leukoc Biol. 95:733. PubMed
  20. Porciello N, et al. 2018. Nat Commun. 9:1080. PubMed
  21. Oyarce C, et al. 2018. Front Immunol. 8:1794. PubMed
  22. Liang F, et al. 2021. RSC Adv. 11:35331. PubMed
  23. Matsumura F, et al. 2021. J Immunol. 207:938. PubMed
  24. Saito S, et al. 2020. Nutrients. 12:. PubMed
  25. Hreha TN, et al. 2020. Front Immunol. 1.597916667. PubMed
  26. Sun T, et al. 2018. Front Immunol. 9:1245. PubMed
  27. Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed
  28. Bode K, et al. 2020. Cell Reports. 29(13):4435-4446.e9.. PubMed
  29. Wang G, et al. 2021. Nat Commun. 12:5733. PubMed
  30. Bommireddy R, et al. 2020. Vaccines (Basel). 8:. PubMed
  31. Dolgova EV, et al. 2022. Int J Mol Sci. 23:. PubMed
  32. Kimura A, et al. 2018. J Exp Med. 215:2197. PubMed
  33. Jiang H, et al. 2018. Front Pharmacol. 9:612. PubMed
  34. Blank A, et al. 2021. J Pathol. 253:160. PubMed
  35. Li X, et al. 2014. PLoS One. 9:108192. PubMed
  36. Lv Z, et al. 2020. Oncoimmunology. 9:1747688. PubMed
  37. Funk KE, et al. 2019. J Neuroinflammation. 16:22. PubMed
  38. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  39. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  40. Kornstädt L, et al. 2021. Front Immunol. 11:607048. PubMed
  41. Pandey V, et al. 2021. eLife. 0.416666666666667. PubMed
  42. Balashov L 2015. Mult Scler. 21: 1131-1138. PubMed
  43. Liang Y, et al. 2014. J Immunol. 192:1277. PubMed
  44. Wang J, et al. 2021. Nat Commun. 12:6198. PubMed
  45. Zhou Y, et al. 2020. Front Pharmacol. 10:1579. PubMed
  46. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  47. Li X, et al. 2009. PLoS One. 4:e6385. PubMed
  48. Cheng Y, et al. 2020. PLoS Pathog. 16:e1008569. PubMed
  49. Zhang C, et al. 2022. Nat Commun. 13:3468. PubMed
  50. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  51. Ji W, et al. 2018. Evid Based Complement Alternat Med. 2017:3272649. PubMed
  52. Xiong W, et al. 2022. Adv Sci (Weinh). 9:e2103029. PubMed
  53. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  54. Gómez LA, et al. 2020. Front Microbiol. 11:1586. PubMed
  55. Benhamron S, et al. 2012. PLoS One. 7:e35602. PubMed
  56. Sanchez M et al. 2017. Cell host & microbe. 22(4):543-551 . PubMed
  57. Cianciaruso C, et al. 2020. Cell Reports. 27(10):3062-3080.e11.. PubMed
  58. Wang Y, et al. 2021. Nat Commun. 12:4964. PubMed
  59. Zhou J, et al. 2022. Int J Oncol. 61: . PubMed
  60. Nechama M, et al. 2018. Nat Commun. 9:1603. PubMed
  61. Xia Y, et al. 2018. Cell. 175:1059. PubMed
  62. Zheng B, et al. 2021. Bioact Mater. 6:3879. PubMed
  63. Wang L, et al. 2021. Sci Adv. 7:eabj4796. PubMed
  64. Jiang L, et al. 2020. Cell. 183(5):1219-1233.e18. PubMed
  65. Alhudaithi SS, et al. 2020. Mol Pharm. 17:4691. PubMed
  66. Sivapatham S, et al. 2019. Front Immunol. 1.865277778. PubMed
  67. Wawrzyniak M, et al. 2021. Pharmacol Res Perspect. 9:e00837. PubMed
  68. Jiang Y, et al. 2021. Nat Commun. 12:742. PubMed
  69. Ren S, et al. 2022. Int J Biol Sci. 18:166. PubMed
  70. Tassi I, et al. 2014. J Immunol. 193:4303. PubMed
  71. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  72. Nicolas-Boluda A, et al. 2021. eLife. 10:00. PubMed
  73. Hao L, et al. 2021. Br J Pharmacol. 178:4726. PubMed
  74. Miao Y et al. 2019. Cell. 177(5):1172-1186 . PubMed
  75. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  76. Funk KE, et al. 2021. Aging Cell. 20:e13412. PubMed
  77. Shi GN, et al. 2021. Ther Adv Med Oncol. 13:1758835920987056. PubMed
  78. Sadiq BA, et al. 2020. Current Protocols in Immunology. 131(1):e115. PubMed
RRID
AB_313126 (BioLegend Cat. No. 104705)
AB_313126 (BioLegend Cat. No. 104706)

Antigen Details

Structure
Ig superfamily, 60 kD
Distribution

Macrophages, activated B cells and T cells, dendritic cells

Function
T cell costimulation
Ligand/Receptor
CD28 (stimulatory), CD152(CTLA4) (inhibitory)
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Linsley PS, et al. 1991. J. Exp. Med. 174:561.
3. Salomon B, et al. 2001. Annu. Rev. Immunol. 19:225.

Gene ID
12519 View all products for this Gene ID
UniProt
View information about CD80 on UniProt.org
Go To Top Version: 2    Revision Date: 02/18/2013

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account