FITC anti-mouse GM-CSF Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Yeast-expressed, recombinant mouse GM-CSF

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)

Application Notes

ELISA or ELISPOT Capture^1,3-5: The purified MP1-22E9 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP1-31G6 antibody (Cat. No. 505502) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture.
Flow Cytometry⁸: The fluorochrome-labeled MP1-22E9 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify GM-CSF -producing cells within mixed cell populations.
Neutralization^2-4: The MP1-22E9 antibody can neutralize the bioactivity of natural or recombinant GM-CSF. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo and in vitro neutralization (Cat. No. 505408).
Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections^1,6,7, and immunocytochemistry⁸.

Application References

(PubMed link indicates BioLegend citation)

1. Sander B, et al. 1993. J. Immunol. Methods 166:201.
2. Suda T, et al. 1990. Cell. Immunol. 129:228.
3. Nozaki S, et al. 1991. J. Invest. Dermatol. 97:10.
4. Abrams JS, et al. 1992. Immunol. Rev. 127:5.
5. Abrams JS. 2001. Curr. Protoc. Immunol. Unit 6.20.
6. Sander B, et al. 1991. Immunol. Rev. 119:65.
7. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
8. Larkin J, et al. 2006. J. Immunol. 177:268.

Product Citations

1. Manivasagam S, et al. 2022. J Immunol. 208:1341. PubMed
2. Vancsik T, et al. 2021. Front Oncol. 590764:11. PubMed
3. Nakagawa S et al. 2017. Cell host & microbe. 22(5):667-677 . PubMed
4. Britton GJ et al. 2019. Immunity. 50(1):212-224 . PubMed
5. Wang F, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:257. PubMed
6. Hernández–Santos N, et al. 2018. Cell Host Microbe. 1.313194444. PubMed
7. Nakajima T, et al. 2021. J Exp Med. 218:. PubMed
8. Lee P, et al. 2014. J Immunol. 192:178. PubMed
9. Wagner A, et al. 2021. Cell. . PubMed
10. Wu B, et al. 2021. Immunity. 54(2):308-323.e6. PubMed
11. Gounni A, et al. 2004. J Immunol. 173:2771. PubMed
12. Larkin J, et al. 2006. J Immunol. 177:268. PubMed
13. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed

RRID

AB_315379 (BioLegend Cat. No. 505403)
AB_315379 (BioLegend Cat. No. 505404)

Antigen Details

Structure

Cytokine; 22 kD (Mammalian)

Bioactivity

Growth/development granulocyte/macrophage progenitors; differentiates myeloblasts/monoblasts; synergizes with Epo proliferation of erythroid/megakaryocytic progenitors

Cell Sources

T cells, monocytes/macrophages, fibroblasts, endothelial cells, mast cells

Cell Targets

Granulocyte/macrophage/erythroid/megakaryocytic progenitors, myeloblasts, monoblasts

Receptors

Heterodimer GM-CSFR α subunit (CDw116); β-subunit (CDw131) in common with IL-3R, IL-5R

Biology Area

Cell Biology, Stem Cells

Molecular Family

Cytokines/Chemokines, Growth Factors

Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Demetri, G., et al. 1991. Blood 78:2791.
3. Fan, D., et al. 1991. In vivo 5:571.
4. Negrin, R., et al. 1992. Adv. Pharmacol. 23:263.

Regulation

Synergistic with IL-1, IL-3, G-CSF; E21R competitive antagonist for receptor binding; stored in ECM with heparan sulfate proteoglycans

Gene ID

12981 View all products for this Gene ID

UniProt

View information about GM-CSF on UniProt.org

Related Protocols

• Surface and Intracellular Cytokine Staining for Flow Cytometry - Video
• Intracellular Flow Cytometry Staining Protocol

Other Formats