FITC anti-mouse IgMa Antibody

Pricing & Availability
Clone
MA-69 (See other available formats)
Regulatory Status
RUO
Other Names
Igh-6a
Isotype
Mouse (C57BL/6) IgG1, κ
DS-1_FITC_052907
  • DS-1_FITC_052907
  • DS-1_FITC_052907_2
    Balb/c (upper) and C57BL/6 (bottom) splenocytes stained with MA-69 FITC
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability
408606 500 µg $329.00
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Description

This antibody was raised by immunization with IgMa mAb TC31. It specifically reacts with mouse IgM of Igh-Ca and the related haplotypes (c, f, g, h, and j). It has been reported that beads coated with MA-69 antibody can stimulate B cell proliferation.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: ELISA as capture antibody or detection antibody, stimulation of B cell proliferation, and immunohistochemistry of acetone-fixed frozen sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Sieckmann D, et al. 1991. Hybridoma 10:121.
  2. Stall AM. 1996. Weir's Handbook of Experimental Immunology pp. 27.
Product Citations
  1. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  2. Brooks JF, et al. 2021. Cell Reports. 36(9):109645. PubMed
  3. Noviski M, et al. 2018. Elife. 7:e35074. PubMed
RRID
AB_940541 (BioLegend Cat. No. 408606)
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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