FITC anti-mouse LAP (TGF-β1) Antibody

Pricing & Availability
Clone
TW7-16B4 (See other available formats)
Regulatory Status
RUO
Other Names
Latency Associated Peptide (LAP), Transforming growth factor-beta 1 (TGF-b1), TGFB1, DPD1, TGF-beta1
Isotype
Mouse IgG1, κ
TW7-16B4_FITC_LAP_Antibody_1_102717
C57BL/6 mouse splenocytes were stimulated with CD3, CD28, and recombinant mouse IL-2 for 48 hours, then surface stained with CD4 Pacific Blue™ and LAP (TGF-β1) (clone TW7-16B4) FITC (top) or mouse IgG1, κ FITC isotype control (bottom). This was followed by intracellular staining with FOXP3 APC. Data shown was generated by gating on CD4+ lymphocyte population.
  • TW7-16B4_FITC_LAP_Antibody_1_102717
    C57BL/6 mouse splenocytes were stimulated with CD3, CD28, and recombinant mouse IL-2 for 48 hours, then surface stained with CD4 Pacific Blue™ and LAP (TGF-β1) (clone TW7-16B4) FITC (top) or mouse IgG1, κ FITC isotype control (bottom). This was followed by intracellular staining with FOXP3 APC. Data shown was generated by gating on CD4+ lymphocyte population.
  • TW7-16B4_FITC_LAP_Antibody_2_102717
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability
141413 25 µg $124.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
141414 100 µg $329.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Transforming growth factor beta (TGF-β) is a cytokine that has critical functions in the immune response by regulating Treg and Th17 cells. TGF-β is first synthesized as pro-TGF-β and then it is cleaved by furin proprotein convertase in the Golgi apparatus to produce the dimeric propeptides called latency-associate peptide (LAP) that non-covalently associates with the dimeric mature TGF-β to prevent its activity. This complex can further associate with latent-TGF-β-binding protein (LTBP) to produce a large latent form for deposition onto the extracellular matrix. The latent-TGF-β can be expressed on the membrane of activated Treg cells, immature dendritic cells, megakaryocytes, and platelets.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse Tgfb1-transduced P3U1 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone TW7-16B4 has been reported to not cross-react with bovine LAP.2 Several anti-LAP antibody clones have been compared and characterized for their LAP reactivity.2 TW7-16B4 recognizes recombinant LAP, latent TGF-ß, and pro-TGF-ß.

Additional reported applications (for relevant formats) include: Western blotting1 and immunoprecipitation1. 

Application References

(PubMed link indicates BioLegend citation)
  1. Oida T, et al. 2010. PLoS One 5:e15523. (FC, IP, WB)
  2. Oida T, et al. 2011. PLoS One 6:e18365. (Neut)
Product Citations
  1. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  2. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  3. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
RRID
AB_2721327 (BioLegend Cat. No. 141413)
AB_2721327 (BioLegend Cat. No. 141414)

Antigen Details

Structure
Dimmers of latency-associated peptide non-covalently associated with dimmers of mature TGF-β
Distribution

Many cell types, highly expressed on activated Tregs and platelets

Function
TGF-β controls cell differentiation, tissue morphogenesis, cell growth, inflammation, matrix synthesis, apoptosis, and regulates immune response
Ligand/Receptor
TGF-β receptors
Cell Type
Dendritic cells, Platelets, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Oida T, et al. 2010. PLoS One 5:e15523.
2. Tran D, et al. 2009. P. Natl. Acad. Sci. USA 106:13445.
3. Ochi H, et al. 2006. Nat. Med. 12:627.
4. Oida T, et al. 2003. J. Immunol. 170:2516.
5. Nakamura K. 2001. J. Exp. Med. 194:629.
6. Miyazono K, et al. 1993. Growth Factors 8:11.

Gene ID
21803 View all products for this Gene ID
UniProt
View information about LAP on UniProt.org
Go To Top Version: 0    Revision Date: 10/31/2017

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account