GMP PE/Cyanine7 anti-human CD25 Antibody

Pricing & Availability
Clone
M-A251 (See other available formats)
Workshop
IV A053
Other Names
IL-2 receptor α chain, Low affinity IL-2R, IL-2Rα chain
Isotype
Mouse IgG1, κ
M-A251_GMP_PE-Cyanine7_CD25_060223
Typical results from human peripheral blood lymphocytes stained either with M-A251 PE/Cyanine7 used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
  • M-A251_GMP_PE-Cyanine7_CD25_060223
    Typical results from human peripheral blood lymphocytes stained either with M-A251 PE/Cyanine7 used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
Compare all formats See GMP PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability
260280 100 tests $440.00
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Description

CD25 is a 55 kD type I transmembrane glycoprotein also known as low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. Soluble CD25/IL-2Rα is produced as a consequence of lymphocyte stimulation and is found in biological fluids following inflammatory responses. CD25 associates with IL-2 receptor β (CD122) and common γ (CD132) chains to form a high affinity IL-2R complex.

Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human PHA-induced lymphocyte cells
Formulation
Phosphate-buffered solution, pH 7.2, containing True-Stain Monocyte Blocker™, 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
100 µg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde fixed frozen sections1 and spatial biology (IBEX)2,3.

The CD25 molecule reveals three epitope regions: A, B, and C. M-A251 antibody recognizes epitope region B. Unlike other CD25 antibody clones, M-A251 can detect CD25 after fixation with paraformaldehyde.

Application References

(PubMed link indicates BioLegend citation)
  1. Li H and Pauza CD. 2015. Eur. J. Immunol. 45:298. (IHC)
  2. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  3. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Disclaimer

GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:

  • Batch-to-batch consistency
  • Material traceability
  • Documented procedures
  • Documented employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • Quality audits per ISO 13485:2016
  • QA review of released products

Antigen Details

Structure
Type I transmembrane glycoprotein, 55 kD; low-affinity IL-2 receptor α chain
Distribution

Activated T and B cells, monocytes/macrophages, Tregs

Interaction
Associates with IL-2Rβ/CD122 and IL-2Rγ/CD132 receptor chains to form a high-affinity IL-2R complex
Ligand/Receptor
IL-2
Cell Type
B cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Knapp W, et al. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press.
2. Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press.
3. Barclay N, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press Inc.
4. Taniguchi T and Minami Y. et al. 1993. Cell 73:5.
5. Waldmann T. 1991. J. Biol. Chem. 266:2681.

Gene ID
3559 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

Other Formats

View All CD25 Reagents Request Custom Conjugation
Description Clone Applications
APC/Cyanine7 anti-human CD25 M-A251 FC
Purified anti-human CD25 M-A251 FC,IHC-F
PE anti-human CD25 M-A251 FC
FITC anti-human CD25 M-A251 FC
PE/Cyanine7 anti-human CD25 M-A251 FC
APC anti-human CD25 M-A251 FC
PerCP/Cyanine5.5 anti-human CD25 M-A251 FC
Brilliant Violet 421™ anti-human CD25 M-A251 FC
Alexa Fluor® 488 anti-human CD25 M-A251 FC
Alexa Fluor® 700 anti-human CD25 M-A251 FC
Brilliant Violet 510™ anti-human CD25 M-A251 FC
PE/Dazzle™ 594 anti-human CD25 M-A251 FC
Biotin anti-human CD25 M-A251 FC
Alexa Fluor® 647 anti-human CD25 M-A251 FC,SB
Pacific Blue™ anti-human CD25 M-A251 FC
PerCP anti-human CD25 M-A251 FC
APC/Fire™ 750 anti-human CD25 M-A251 FC
Brilliant Violet 711™ anti-human CD25 M-A251 FC
Brilliant Violet 785™ anti-human CD25 M-A251 FC
Brilliant Violet 605™ anti-human CD25 M-A251 FC
KIRAVIA Blue 520™ anti-human CD25 M-A251 FC
PE/Fire™ 700 anti-human CD25 M-A251 FC
APC/Fire™ 810 anti-human CD25 M-A251 FC
Spark NIR™ 685 anti-human CD25 Antibody M-A251 FC
Spark YG™ 581 anti-human CD25 M-A251 FC
PE/Fire™ 640 anti-human CD25 Antibody M-A251 FC
PE anti-human CD25 M-A251 FC
PerCP/Cyanine5.5 anti-human CD25 M-A251 FC
APC/Fire™ 750 anti-human CD25 M-A251 FC
PE/Cyanine7 anti-human CD25 M-A251 FC
APC anti-human CD25 M-A251 FC
PE/Cyanine5 anti-human CD25 M-A251 FC
FITC anti-human CD25 M-A251 FC
Spark Red™ 718 anti-human CD25 M-A251 FC
GMP PE anti-human CD25 M-A251 FC
GMP PE/Cyanine7 anti-human CD25 M-A251 FC
PerCP/Fire™ 780 anti-human CD25 M-A251 FC
PE/Fire™ 744 anti-human CD25 M-A251 FC
PerCP/Fire™ 806 anti-human CD25 M-A251 FC
Brilliant Violet 650™ anti-human CD25 M-A251 FC
Brilliant Violet 750™ anti-human CD25 M-A251 FC
Go To Top Version: 1    Revision Date: 06/19/2023

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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