Pacific Blue™ anti-human CD3 Antibody

Pricing & Availability
Clone
UCHT1 (See other available formats)
Regulatory Status
RUO
Workshop
III 471
Other Names
T3, CD3ε
Isotype
Mouse IgG1, κ
UCHT1_Blue_022008
Human peripheral blood lymphocytes stained with UCHT1 Pacific Blue™
  • UCHT1_Blue_022008
    Human peripheral blood lymphocytes stained with UCHT1 Pacific Blue™
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability
300418 25 µg $112.00
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300431 100 tests $264.00
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300417 100 µg $268.00
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300442 500 tests $1188.00
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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
µg sizes: 0.5 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The CD3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
For test size, the suggested use of this reagent for immunofluorescent staining is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
For µg sizes, the suggested use of this reagent for immunofluorescent staining is ≤2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood.
It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
  2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
  3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
  4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
  5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
  6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
  7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
  12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
  13. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  14. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  15. Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Schnizer C 2022. Frontiers in Immunology. 13:1095129. PubMed
  2. Yen M, et al. 2022. Cell. 185:1414. PubMed
  3. Liu Y, et al. 2023. Nat Commun. 14:2179. PubMed
  4. Weymar GHJ, et al. 2022. Cell Rep. 40:111311. PubMed
  5. Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
  6. Leach SM, et al. 2020. Cell Rep. 33:108337. PubMed
  7. Kerkman PF, Kempers AC 2016. Ann Rheum Dis. 75(12):2201-2207. PubMed
  8. Manser AR, et al. 2019. J Immunol. 203:2301. PubMed
  9. Yarzabek B et al. 2018. eLife. 7 pii: e34961. PubMed
  10. Leite NC, et al. 2020. Cell Reports. 32(2):107894.. PubMed
  11. Geng J, et al. 2018. Elife. 7:e36341. PubMed
  12. Kraig E, et al. 2018. Exp Gerontol. 105:53. PubMed
  13. Japp AS, et al. 2021. Cell. 184(3):827-839.e14. PubMed
  14. Alter G, et al. 2020. Cell. 183(1):185-196.e14. PubMed
  15. Tu HA, et al. 2020. Cell Rep Med. 1:100155. PubMed
  16. Tjwa E, et al. 2011. J Hepatol. 54:209. PubMed
  17. Ye C, et al. 2017. J Virol. 91:e01389-23. PubMed
  18. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  19. Fu W, et al. 2016. Sci Rep. 6:38162. PubMed
  20. Laroni A, et al. 2016. J Autoimmun. 72:8-18. PubMed
  21. Bego M, et al. 2015. J Vis Exp. 103: 51207. PubMed
  22. Hesketh AJ et al. 2015. PloS one. 10(12):e0145197 . PubMed
  23. Schoutrop E, et al. 2022. Oncoimmunology. 11:2093426. PubMed
  24. Glassman CR, et al. 2021. Cell. 184(4):983-999.e24. PubMed
  25. Gawel DR, et al. 2019. Genome Med. 11:47. PubMed
  26. Barman S, et al. 2016. Int Immunol. 28: 533 - 545. PubMed
  27. Horwitz JA, et al. 2017. Cell. 170:637. PubMed
  28. Schneider EH, et al. 2020. Naunyn Schmiedebergs Arch Pharmacol. 393:1251. PubMed
  29. Roy Chowdhury R, et al. 2018. Nature. 560:644. PubMed
  30. Rydbirk R, et al. 2019. Sci Rep. 9:7781. PubMed
  31. Bensberg M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  32. Wang R, et al. 2016. Proc Natl Acad Sci U S A. 113: 11501 - 11506. PubMed
  33. Yang C, et al. 2019. Nat Commun. 10:3931. PubMed
  34. Artinger M, et al. 2021. Int J Mol Sci. 22: . PubMed
  35. Assadi G, et al. 2016. PLoS One. 11:e0168276. PubMed
  36. Mock U, et al. 2015. Nucleic Acids Res. 43:5560. PubMed
  37. Richard J, et al. 2013. Virology. 443:248. PubMed
  38. Grant EJ, et al. 2018. Nat Commun. 9:5427. PubMed
RRID
AB_2562048 (BioLegend Cat. No. 300418)
AB_2562048 (BioLegend Cat. No. 300431)
AB_2562048 (BioLegend Cat. No. 300417)
AB_2562048 (BioLegend Cat. No. 300442)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD3 UCHT1 FC
Biotin anti-human CD3 UCHT1 FC
FITC anti-human CD3 UCHT1 FC
PE anti-human CD3 UCHT1 FC
PE/Cyanine5 anti-human CD3 UCHT1 FC
Purified anti-human CD3 UCHT1 FC,CyTOF®,IHC-F,IP,Activ,WB
Alexa Fluor® 647 anti-human CD3 UCHT1 FC,ICC,IHC-F
Alexa Fluor® 488 anti-human CD3 UCHT1 FC,ICC,IHC-F
Pacific Blue™ anti-human CD3 UCHT1 FC
PE/Cyanine7 anti-human CD3 UCHT1 FC
Alexa Fluor® 700 anti-human CD3 UCHT1 FC
APC/Cyanine7 anti-human CD3 UCHT1 FC
PerCP anti-human CD3 UCHT1 FC
PerCP/Cyanine5.5 anti-human CD3 UCHT1 FC
Brilliant Violet 421™ anti-human CD3 UCHT1 FC,ICC,IHC-F
Brilliant Violet 570™ anti-human CD3 UCHT1 FC
Ultra-LEAF™ Purified anti-human CD3 UCHT1 FC,CyTOF®,IHC-F,IP,Activ,WB
Purified anti-human CD3 (Maxpar® Ready) UCHT1 FC,CyTOF®
Alexa Fluor® 594 anti-human CD3 UCHT1 ICC,FC,SB
PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
Brilliant Violet 510™ anti-human CD3 UCHT1 FC
Brilliant Violet 605™ anti-human CD3 UCHT1 FC
Brilliant Violet 711™ anti-human CD3 UCHT1 FC
Brilliant Violet 650™ anti-human CD3 UCHT1 FC
APC/Fire™ 750 anti-human CD3 UCHT1 FC
Pacific Blue™ anti-human CD3 UCHT1 FC
Brilliant Violet 785™ anti-human CD3 UCHT1 FC
PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
TotalSeq™-A0034 anti-human CD3 UCHT1 PG
TotalSeq™-B0034 anti-human CD3 UCHT1 PG
TotalSeq™-C0034 anti-human CD3 UCHT1 PG
PE anti-human CD3 UCHT1 FC
PE/Cyanine7 anti-human CD3 UCHT1 FC
KIRAVIA Blue 520™ anti-human CD3 UCHT1 FC
Spark Violet™ 538 anti-human CD3 Antibody UCHT1 FC
TotalSeq™-D0034 anti-human CD3 UCHT1 PG
Spark Blue™ 574 anti-human CD3 Antibody UCHT1 FC
GMP Pacific Blue™ anti-human CD3 UCHT1 FC
GMP PE anti-human CD3 UCHT1 FC
GMP PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
Spark Violet™ 423 anti-human CD3 UCHT1 FC
GMP PE/Cyanine7 anti-human CD3 UCHT1 FC
Spark Blue™ 515 anti-human CD3 UCHT1 FC
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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