Pacific Blue™ anti-human IgD Antibody

Pricing & Availability
Clone
W18340F (See other available formats)
Regulatory Status
RUO
Other Names
Ig delta chain C region
Isotype
Rat IgG2a, κ
W18340F_PB_IgD_Antibody_111723
Human peripheral blood lymphocytes were stained with anti-human CD19 FITC and anti-human IgD (clone W18340F) Pacific Blue™ (left) or rat IgG2a, к Pacific Blue™ isotype control (right).
  • W18340F_PB_IgD_Antibody_111723
    Human peripheral blood lymphocytes were stained with anti-human CD19 FITC and anti-human IgD (clone W18340F) Pacific Blue™ (left) or rat IgG2a, к Pacific Blue™ isotype control (right).
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability
307825 25 tests $150.00
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307826 100 tests $340.00
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Description

IgD, a member of the immunoglobulin (Ig) family, is expressed in naïve B cells. It has 3 Ig-like domains and exists in a transmembrane and a soluble form. In general, IgD is not secreted and usually its expression is lost after the Ig isotype switch. After antigen binding, IgD signals through the CD79a/CD79b (Igα/Igβ) heterodimer, resulting in the activation of the B cell. (BioLegend Website)

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Native human IgD protein (full length)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. 


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

This clone does not cross react with mouse tissues.

RRID
AB_3097322 (BioLegend Cat. No. 307825)
AB_3097322 (BioLegend Cat. No. 307826)

Antigen Details

Distribution

Naïve B cells

Function
Antigen binding, B cell activation
Cell Type
B cells
Biology Area
Immunology
Antigen References
  1. Geisberger R, et al. 2006. Immunology. 118:429-37.
  2. Weller S, et al. 2005. Eur J Immunol. 35:2789-92.
  3. Brandtzaeg P & Johansen FE. 2005. Immunol Rev. 206:32-63.
Gene ID
3495 View all products for this Gene ID
UniProt
View information about IgD on UniProt.org
Go To Top Version: 1    Revision Date: 11/17/2023

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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