Pacific Blue™ anti-mouse CD317 (BST2, PDCA-1) Antibody

Pricing & Availability
Clone
927 (See other available formats)
Regulatory Status
RUO
Other Names
BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, PDCA-1
Isotype
Rat IgG2b, κ
A_927_PB_011310
C57BL/6 splenocytes stained with 927 Pacific Blue™ and CD45R/B220 (RA3-6B2) PE
  • A_927_PB_011310
    C57BL/6 splenocytes stained with 927 Pacific Blue™ and CD45R/B220 (RA3-6B2) PE
  • B_927_PB_011310
    C57BL/6 splenocytes stained with rat IgG2b Pacific Blue™ isotype control and CD45R/B220 (RA3-6B2) PE
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability
127017 25 µg $147.00
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127018 100 µg $347.00
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Description

CD317, known as BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, or PDCA-1, is type II transmembrane glycoprotein with a molecular mass of 29-33 kD. It is predominantly expressed on Type I IFN-producing cells (IPCs) in naïve mice, but is up-regulated on most cell types following stimulation with type I IFNs and IFN-gamma. It is highly expressed on terminally differentiated normal plasmacytoid dendritic cells and some tumor cells, such as multiple myeloma, renal cell carcinoma, and melanoma cells. BST2 is a recently identified, IFN-induced cellular response factor that blocks release of HIV-1 and other retroviruses from infected cells. BST2 has been found to be the natural ligand of ILT7 in human model.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse plasmacytoid dendritic cells (DCs)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy, functional assay2, and depletion3,4. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays.

Application References

(PubMed link indicates BioLegend citation)
  1. Blasius AL, et al. 2006. J. Immunol. 177:3260.
  2. Schliemann C, et al. 2010. Blood 115:736. (FA, IF)
  3. Rajagopal D, et al. 2010. Blood 115:1949. (Depletion)
  4. Moniz RJ, et al. 2010. FEMS Immunol. Med. Microbiol. 58:397. (Depletion)
  5. Chen YL, et al. 2013. J Exp Med. 210:2515. PubMed
Product Citations
  1. Kobayashi S, et al. 2019. J Immunol. 203:1447. PubMed
  2. Lucas B, et al. 2020. Nat Commun. 11:2198. PubMed
  3. Urata S, et al. 2018. PLoS Pathog. 14:e1007172. PubMed
RRID
AB_1967104 (BioLegend Cat. No. 127017)
AB_1967104 (BioLegend Cat. No. 127018)

Antigen Details

Structure
Type II transmembrane glycoprotein with a molecular mass of 29-33 kD.
Distribution

Expressed on type I IFN-producing cells, plasmacytoid dendritic cells, and neoplastic B cells, such as multiple myeloma.

Function
Recently identified antiviral protein that blocks the release of nascent retrovirus or other particles from infected cells.
Cell Type
Dendritic cells
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Douglas JL. et al. 2009. J Virol. 83(16):7931
2. Cao W et al. 2009. J. Exp. Med. 206(7):1603
3. Neil SJ. et al. 2008. Nature 451:425

Gene ID
69550 View all products for this Gene ID
UniProt
View information about CD317 on UniProt.org
Go To Top Version: 3    Revision Date: 03/04/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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