Pacific Blue™ anti-mouse CD45.1 Antibody

Pricing & Availability
Clone
A20 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5.1, LCA
Isotype
Mouse (A.SW) IgG2a, κ
A20_Blue_020608
SJL mouse splenocytes stained with A20 Pacific Blue™
  • A20_Blue_020608
    SJL mouse splenocytes stained with A20 Pacific Blue™
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability
110721 25 µg $94.00
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110722 100 µg $215.00
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Description

CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The CD45.1 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application Notes

The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).

Application References

(PubMed link indicates BioLegend citation)
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  4. Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
  5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
  6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
  7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
  8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
  9. Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
  10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
  11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
  12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
  13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
  14. Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed
  15. Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
  16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
  17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
  18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  19. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Sandu I, et al. 2020. Nat Commun. 11:4454. PubMed
  2. MacLean AJ, et al. 2022. Immunity. 55:718. PubMed
  3. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  4. Koutník J, et al. 2022. Front Immunol. 13:1049033. PubMed
  5. Lombard-Vadnais F, et al. 2023. iScience. 26:105852. PubMed
  6. Kasuya T, et al. 2023. Sci Rep. 13:1653. PubMed
  7. Cheng H, et al. 2023. Nat Metab. 5:314. PubMed
  8. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  9. Le DT, et al. 2023. iScience. 26:106059. PubMed
  10. Ximerakis M, et al. 2023. Nat Aging. 3:327. PubMed
  11. Roco JA et al. 2019. Immunity. 51(2):337-350 . PubMed
  12. Page N, et al. 2018. Immunity. 48:937. PubMed
  13. Romain Bouziat et al. 2018. Cell host & microbe. 24(5):677-688 . PubMed
  14. Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed
  15. Castiello MC, et al. 2020. J Allergy Clin Immunol. . PubMed
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  17. Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed
  18. Lin G, et al. 2013. J Immunol. 190:4627. PubMed
  19. Jing Li et al. 2018. Immunity. 48(4):773-786 . PubMed
  20. Kosack L, et al. 2017. Sci Rep. 7:11289. PubMed
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  22. Müller M, et al. 2021. Nat Commun. 12:7036. PubMed
  23. Kitano M, et al. 2016. Proc Natl Acad Sci U S A. 113: 1044 - 1049. PubMed
  24. Li L, et al. 2010. J Immunol. 184:1728. PubMed
  25. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
  26. Wigton E, et al. 2016. J Leukoc Biol. 100: 143 - 153. PubMed
  27. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  28. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  29. Onodera T, et al. 2016. J Immunol. 196: 4172 - 4184. PubMed
  30. Lu X, et al. 2016. Nat Commun. 7: 12719. PubMed
  31. Cheng Y, et al. 2021. Cancer Cell. . PubMed
  32. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  33. Kalinski AL, et al. 2020. Elife. 9:00. PubMed
  34. Siggs O, et al. 2015. Proc Natl Acad Sci U S A. 112: E5706 - E5714. PubMed
  35. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  36. Minamitani T, et al. 2015. Proc Natl Acad Sci U S A. 112: 11612-11617. PubMed
  37. Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed
  38. Liu X, et al. 2020. Cell Host Microbe. 28(5):683-698.e6. PubMed
  39. Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed
  40. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  41. Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed
  42. Holian C 2007. Am J Respir Cell Mol Biol. 37:729. PubMed
  43. Nadrah K, et al. 2015. PLoS One. 10: 0136061. PubMed
  44. Florez MA, et al. 2020. Cell Rep. 33:108530. PubMed
  45. Hormaechea-Agulla D, et al. 2021. Cell Stem Cell. . PubMed
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  47. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
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  50. Mills R, et al. 2015. J Immunol. 194:3675. PubMed
  51. Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed
  52. Hamilton J,et al. 2017. J Immunol. 10.4049/jimmunol.1700888. PubMed
  53. Fiege JK, et al. 2019. J Immunol. 203(4):936-945. PubMed
  54. Sabouri Z, et al. 2016. Nat Commun. 7:13381. PubMed
  55. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  56. Diao J, et al. 2018. J Immunol. 201:1306. PubMed
  57. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  58. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  59. Wünsche P et al. 2018. Cell stem cell. 23(1):132-146 . PubMed
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RRID
AB_492866 (BioLegend Cat. No. 110721)
AB_492866 (BioLegend Cat. No. 110722)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.1 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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