Pacific Blue™ anti-mouse CD62L Antibody

Pricing & Availability
Clone
MEL-14 (See other available formats)
Regulatory Status
RUO
Other Names
L-selectin, LECAM-1, Ly-22, LAM-1, MEL-14
Isotype
Rat IgG2a, κ
MEL-14_PB_1_110210
C57BL/6 mouse splenocytes were stained with CD3 Alexa Fluor® 488 and CD62L (clone MEL-14) Pacific Blue™ (top) or rat IgG2a Pacific Blue™ isotype control (bottom).
  • MEL-14_PB_1_110210
    C57BL/6 mouse splenocytes were stained with CD3 Alexa Fluor® 488 and CD62L (clone MEL-14) Pacific Blue™ (top) or rat IgG2a Pacific Blue™ isotype control (bottom).
  • MEL-14_PB_2_110210
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Cat # Size Price Quantity Check Availability
104423 25 µg $101.00
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104424 100 µg $229.00
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Description

CD62L is a 74-95 kD glycoprotein also known as L-selectin, LECAM-1, Ly-22, LAM-1, and MEL-14. It is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte "rolling" on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naïve, effector, and memory T cells. CD62L has been reported to interact with CD34, GlyCAM-1, and MAdCAM-1.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/eb mouse B lymphoma 38C-13
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, complement-dependent cytotoxicity4, in vivo and in vitro blocking of adhesion1-3,5, and immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections6. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104457-104462).

Application References

(PubMed link indicates BioLegend citation)
  1. Gallatin WM, et al. 1983. Nature 304:30. (IP, Block)
  2. Siegelman MH, et al. 1990. Cell 61:611. (IP, Block)
  3. Lewinsohn DM, et al. 1987. J. Immunol. 138:4313. (IP, Block)
  4. Iwabuchi K, et al. 1991. Immunobiology 182:161. (CMCD)
  5. Pizcueta P, et al. 1994. Am. J. Pathol. 145:461.
  6. Reichert RA, et al. 1986. J. Immunol. 136:3535. (IHC, FC)
  7. Olver S, et al. 2006. Cancer Res. 66:571.
  8. Fukushima A, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:657. PubMed
  9. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Lee JW, et al. 2006. Nature Immunol. 8:181.
  12. Shigeta A, et al. 2008. Blood 112:4915 (FC) PubMed
  13. de Vries VC, et al. 2009. Am. J. Transplant. 9:2270 PubMed
Product Citations
  1. Wu B, et al. 2023. J Immunother Cancer. 11: . PubMed
  2. Zhuang WR, et al. 2023. Nat Commun. 14:1675. PubMed
  3. Lv H, et al. 2020. Cell Metabolism. 33(1):110-127.e5. PubMed
  4. Freeley S, et al. 2013. Ann Rheum Dis. 72:1053. PubMed
  5. Biram A, et al. 2020. Cell Rep. 30:1910. PubMed
  6. Ngamsri KC, et al. 2021. Int J Mol Sci. 22:. PubMed
  7. Flommersfeld S, et al. 2021. Immunity. :. PubMed
  8. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  9. Kurniawan H, et al. 2020. Cell Metabolism. 31(5):920-936. PubMed
  10. Ren G et al. 2017. Molecular cell. 67(6):1049-1058 . PubMed
  11. Jiang Y, et al. 2020. Adv Mater. 32:e2001808. PubMed
  12. Daneshmandi S, et al. 2021. Elife. 10:. PubMed
  13. Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed
  14. Miyauchi E, et al. 2020. Nature. 585:102. PubMed
  15. Römer C et al. 2015. The Journal of Neuroscience. 35(20):7777-94 . PubMed
  16. Daneshmandi S, et al. 2021. Cell Reports. 34(10):108831. PubMed
  17. Sun X, et al. 2021. Nature. 599:673. PubMed
  18. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  19. Wu B, et al. 2022. Nat Commun. 13:2155. PubMed
  20. Fleskens V, et al. 2019. Cell Rep. 26:3600. PubMed
RRID
AB_493380 (BioLegend Cat. No. 104423)
AB_493380 (BioLegend Cat. No. 104424)

Antigen Details

Structure
Selectin, 95 kD (neutrophils) or 74 kD (lymphocytes)
Distribution

Subsets of B and T cells, monocytes, granulocytes, subset of NK cells

Function
Lymphocyte homing to HEV, rolling on activated endothelium
Ligand/Receptor
CD34, GlyCAM-1, MAdCAM-1
Cell Type
B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kishimoto TK, et al. 1990. P. Natl. Acad. Sci. USA 87:2244.
3. Tedder TF, et al. 1995. J. Exp. Med. 181:2259.

Gene ID
20343 View all products for this Gene ID
UniProt
View information about CD62L on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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