PE anti-human β2-microglobulin Antibody

Pricing & Availability
Clone
A17082E (See other available formats)
Regulatory Status
RUO
Other Names
β2M, β2-M, beta2-microglobulin b2-M, b2M
Isotype
Mouse IgG1, κ
A17082E_PE_B2-microglobulin_Antibody_122618
Human peripheral blood lymphocytes were stained with β2-microglobulin (clone A17082E) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • A17082E_PE_B2-microglobulin_Antibody_122618
    Human peripheral blood lymphocytes were stained with β2-microglobulin (clone A17082E) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
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395803 25 tests $153.00
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395804 100 tests $341.00
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Description

β2-microglobulin (β2M) is a 12 kD nonpolymorphic Ig like protein. It is a non-membrane-anchored glycoprotein and is noncovalently associated with 39-44 kD polymorphic heavy chains of MHC class I molecules to form HLA class I antigen complex. In association with HLA class I, β2M is expressed on all leukocytes, platelets, endothelial cells, and epithelial cells. β2M plays an essential role both in governing MHC class I molecules stability and in promoting antigen binding and presenting the antigen to CD3/TCR complex of CD8+ T cells.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Beta2-microglobulin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Based on in-house testing, staining for clone A17082E is not blocked by clone W6/32 (anti-HLA-A,B,C) and is only partially blocked by clone 2M2 also raised against human ß2-microglobulin.

RRID
AB_2783319 (BioLegend Cat. No. 395803)
AB_2783319 (BioLegend Cat. No. 395804)

Antigen Details

Structure
12KD, Ig like structure
Distribution

Leukocytes, platelets, endothelial cells, epithelial cells and other nucleated cells.

Function
Form MHC class I
Interaction
MHC class I heavy chain and other MHC I like molecules.
Cell Type
Endothelial cells, Epithelial cells, Leukocytes, Platelets
Antigen References
  1. Engelhard VH. 1994. Curr Opin Immunol. 6:13.
  2. Williams DB, et al. 1989. J Immunol. 142:2796.
  3. Danliczyk UG and TL. Delovitch. 1994. J Immunol. 153:3533.
  4. Williams A, et al. 2002. Tissue Antigens. 59:3.
Gene ID
567 View all products for this Gene ID
UniProt
View information about beta2-microglobulin on UniProt.org
Go To Top Version: 1    Revision Date: 12/26/2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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