- Clone
- A17082E (See other available formats)
- Regulatory Status
- RUO
- Other Names
- β2M, β2-M, beta2-microglobulin b2-M, b2M
- Isotype
- Mouse IgG1, κ
-
Human peripheral blood lymphocytes were stained with ß2-microglobulin (clone A17082E) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 395809 | 25 tests | $176.00 | |||
| 395810 | 100 tests | $382.00 | |||
β2-microglobulin (β2M) is a 12 kD nonpolymorphic Ig like protein. It is a non-membrane-anchored glycoprotein and is noncovalently associated with 39-44 kD polymorphic heavy chains of MHC class I molecules to form HLA class I antigen complex. In association with HLA class I, β2M is expressed on all leukocytes, platelets, endothelial cells, and epithelial cells. β2M plays an essential role both in governing MHC class I molecules stability and in promoting antigen binding and presenting the antigen to CD3/TCR complex of CD8+ T cells.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human Beta2-microglobulin
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. - Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
Based on in-house testing, staining for clone A17082E is not blocked by clone W6/32 (anti-HLA-A,B,C) and is only partially blocked by clone 2M2 also raised against human ß2-microglobulin.
- RRID
-
AB_2810595 (BioLegend Cat. No. 395809)
AB_2810595 (BioLegend Cat. No. 395810)
Antigen Details
- Structure
- 12KD, Ig like structure
- Distribution
-
Leukocytes, platelets, endothelial cells, epithelial cells and other nucleated cells.
- Function
- Form MHC class I
- Interaction
- MHC class I heavy chain and other MHC I like molecules.
- Cell Type
- Endothelial cells, Epithelial cells, Leukocytes, Platelets
- Antigen References
-
- Engelhard VH. 1994. Curr Opin Immunol. 6:13.
- Williams DB, et al. 1989. J Immunol. 142:2796.
- Danliczyk UG and TL. Delovitch. 1994. J Immunol. 153:3533.
- Williams A, et al. 2002. Tissue Antigens. 59:3.
- Gene ID
- 567 View all products for this Gene ID
- UniProt
- View information about beta2-microglobulin on UniProt.org
Other Formats
View All beta2-microglobulin Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-human β2-microglobulin | A17082E | FC |
| APC anti-human β2-microglobulin | A17082E | FC |
| PerCP/Cyanine5.5 anti-human β2-microglobulin | A17082E | FC |
| APC/Fire™ 750 anti-human β2-microglobulin | A17082E | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human β2-microglobulin
Human peripheral blood lymphocytes were stained with β2-micr... -
APC anti-human β2-microglobulin
Human peripheral blood lymphocytes were stained with β2-micr... -
PerCP/Cyanine5.5 anti-human β2-microglobulin
Human peripheral blood lymphocytes were stained with ß2-micr... -
APC/Fire™ 750 anti-human β2-microglobulin
Human peripheral blood lymphocytes were stained with β2-micr...