PE anti-human CD117 (c-kit) Antibody

Pricing & Availability
Clone
104D2 (See other available formats)
Regulatory Status
RUO
Other Names
Stem cell factor receptor, c-kit, mast cell growth factor receptor, steel factor receptor
Isotype
Mouse IgG1, κ
1_104D2_PE_033007
Human erythroleukemia cell line TF-1 stained with 104D2 PE
  • 1_104D2_PE_033007
    Human erythroleukemia cell line TF-1 stained with 104D2 PE
  • 2_30_Human_Skin_CD117_CD138_Vimentin
    Confocal image of human skin sample acquired using the IBEX method of highly multiplexed antibody-based imaging: CD117 (magenta) in Cycle 3, CD138 (cyan) in Cycle 4, and Vimentin (blue) in Cycle 5. Tissues were prepared using ~1% (vol/vol) formaldehyde and a detergent. Following fixation, samples are immersed in 30% (wt/vol) sucrose for cryoprotection. Images are courtesy of Drs. Andrea J. Radtke and Ronald N. Germain of the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
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313203 25 tests $108.00
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313204 100 tests $232.00
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Description

CD117 is a 145 kD protein tyrosine kinase also known as c-Kit. It is a receptor for stem cell factor or c-Kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemia cells (AML). CD117 binding of c-Kit ligand induces phosphorylation of CD117 and stimulates proliferation and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulo-monocytic committed cells.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus, Cow
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
MOLM-1 megakaryocytic cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The CD117 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
SB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The 104D2 antibody does not block binding of c-Kit ligand. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy1, and spatial biology (IBEX)4,5.

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References

(PubMed link indicates BioLegend citation)
  1. Broudy VC, et al. 1999. Blood 94:1979. (IF, IP)
  2. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  3. Nagano M, et al. 2007. Blood 110:151. (FC) PubMed
  4. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Matkovic Leko I, et al. 2023. Nat Protoc. . PubMed
  2. Sun J, et al. 2008. J Biol Chem. 283:27444. PubMed
  3. Sun J, et al. 2018. Oncogenesis. 7:48. PubMed
  4. Zhao Y, et al. 2016. Cell Rep. 16:2003-2016. PubMed
  5. Sun J, et al. 2009. J Biol Chem. 284:11039. PubMed
  6. Niwa A, et al. 2011. PLoS One. 6:e22261. PubMed
  7. Cho K, et al. 2017. Biochem Biophys Res Commun. 10.1016/j.bbrc.2017.04.141. PubMed
  8. Taguchi A, et al. 2017. Cell Stem Cell. 21:730. PubMed
  9. Cruse G, et al. 2015. Mol Biol Cell. 26:1711. PubMed
  10. Lecluze E, et al. 2020. Hum Reprod. 35:1099. PubMed
  11. Shen C, et al. 2021. Front Immunol. 12:680055. PubMed
  12. Zhang J, et al. 2019. Dev Cell. 48:329. PubMed
  13. Schielke L, et al. 2022. Front Immunol. 13:916701. PubMed
  14. Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
  15. Suzuki S, et al. 2021. STAR Protocols. 2:100683. PubMed
  16. Zeng Z, et al. 2021. Nat Commun. 12:3641. PubMed
  17. Smith-Díaz CC, et al. 2021. Front Oncol. 11:709543. PubMed
  18. López-Sanz C, et al. 2022. STAR Protoc. 3:101755. PubMed
  19. Bender R, et al. 2016. PLoS Pathog. 12: 1005641. PubMed
  20. Zhang Y et al. 2018. Cell stem cell. 23(4):516-529 . PubMed
  21. Pan C, et al. 2019. Int J Mol Med. 44:1629. PubMed
  22. Mpakou V, et al. 2021. Exp Ther Med. 1.010416667. PubMed
  23. Samuel RM, et al. 2020. Cell Stem Cell. 27:876. PubMed
  24. Bennstein SB, et al. 2020. eLife. 9:e55232.. PubMed
  25. Wang G, et al. 2018. Int J Mol Med. 41:791. PubMed
RRID
AB_314982 (BioLegend Cat. No. 313203)
AB_314982 (BioLegend Cat. No. 313204)

Antigen Details

Structure
Growth factor receptor with tyrosine kinase activity, subclass III, approximately 145 kD
Distribution

Pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, acute myeloid leukemic cells (AML)

Function
Growth factor receptor for stem cell factor. Induces proliferation and survival of primitive hematopoietic progenitors. Potent inducer of proliferation in erythroid-committed progenitor cells. Defects in CD117 have been linked to severe anemia and a decreased number of hematopoietic progenitor cells.
Ligand/Receptor
c-Kit ligand
Modification
Multiple phosphorylation sites
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Giebel LB, et al. 1992. Oncogene 7:2207.
2. Furitsu T, et al. 1993. J. Clin. Invest. 92:1736.

Gene ID
3815 View all products for this Gene ID
UniProt
View information about CD117 on UniProt.org

Other Formats

View All CD117 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CD117 (c-kit) 104D2 FC,CyTOF®,ICC,IP
PE anti-human CD117 (c-kit) 104D2 FC,SB
APC anti-human CD117 (c-kit) 104D2 FC
Biotin anti-human CD117 (c-kit) 104D2 FC
PE/Cyanine5 anti-human CD117 (c-kit) 104D2 FC
PE/Cyanine7 anti-human CD117 (c-kit) 104D2 FC
PerCP/Cyanine5.5 anti-human CD117 (c-kit) 104D2 FC
Brilliant Violet 421™ anti-human CD117 (c-kit) 104D2 FC
Brilliant Violet 605™ anti-human CD117 (c-kit) 104D2 FC
Brilliant Violet 510™ anti-human CD117 (c-kit) 104D2 FC
Brilliant Violet 650™ anti-human CD117 (c-kit) 104D2 FC
Purified anti-human CD117 (c-kit) (Maxpar® Ready) 104D2 FC,CyTOF®
PE/Dazzle™ 594 anti-human CD117 (c-kit) 104D2 FC
APC/Cyanine7 anti-human CD117 (c-kit) 104D2 FC
Brilliant Violet 711™ anti-human CD117 (c-kit) 104D2 FC
FITC anti-human CD117 (c-kit) 104D2 FC
Alexa Fluor® 488 anti-human CD117 (c-kit) 104D2 FC,SB
Alexa Fluor® 647 anti-human CD117 (c-kit) 104D2 FC
APC/Fire™ 750 anti-human CD117 (c-kit) 104D2 FC
Brilliant Violet 785™ anti-human CD117 (c-kit) 104D2 FC
PE anti-human CD117 (c-kit) 104D2 FC
APC anti-human CD117 (c-kit) 104D2 FC
TotalSeq™-A0061 anti-human CD117 (c-kit) 104D2 PG
TotalSeq™-C0061 anti-human CD117 (c-kit) 104D2 PG
TotalSeq™-B0061 anti-human CD117 (c-kit) 104D2 PG
Alexa Fluor® 700 anti-human CD117 (c-kit) 104D2 FC
Spark NIR™ 685 anti-human CD117 (c-kit) Antibody 104D2 FC
APC/Fire™ 750 anti-human CD117 (c-kit) 104D2 FC
TotalSeq™-D0061 anti-human CD117 (c-kit) 104D2 PG
PE/Cyanine7 anti-human CD117 (c-kit) 104D2 FC
PE/Dazzle™ 594 anti-human CD117 (c-kit) 104D2 FC
PerCP/Cyanine5.5 anti-human CD117 (c-kit) 104D2 FC
GMP APC anti-human CD117 (c-kit) 104D2 FC
GMP PE anti-human CD117 (c-kit) 104D2 FC
Spark Red™ 718 anti-human CD117 (c-kit) (Flexi-Fluor™) 104D2 FC
Go To Top Version: 4    Revision Date: 04/18/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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