- Clone
- 104D2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Stem cell factor receptor, c-kit, mast cell growth factor receptor, steel factor receptor
- Isotype
- Mouse IgG1, κ
- Barcode Sequence
- AGACTAATAGCTGAC
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 313247 | 10 µg | $369.00 | |||
CD117 is a 145 kD protein tyrosine kinase also known as c-Kit. It is a receptor for stem cell factor or c-Kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemia cells (AML). CD117 binding of c-Kit ligand induces phosphorylation of CD117 and stimulates proliferation and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulo-monocytic committed cells.
Product Details
- Verified Reactivity
- Human
- Reported Reactivity
- Cynomolgus, Cow
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- MOLM-1 megakaryocytic cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
- Preparation
- The antibody was purified by chromatography and conjugated with TotalSeq™-B oligomer under optimal conditions.
- Concentration
- 0.5 mg/mL
- Application
-
PG - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-B antibodies are compatible with 10x Genomics Single Cell Gene Expression Solutions.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform. - Application Notes
-
The 104D2 antibody does not block binding of c-Kit ligand. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy1, and spatial biology (IBEX)4,5.
- Additional Product Notes
-
TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.
The barcode flanking sequences are GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTNNNNNNNNNN (PCR handle), and NNNNNNNNNGCTTTAAGGCCGGTCCTAGC*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.
View more applications data for this product in our Scientific Poster Library. -
Application References
(PubMed link indicates BioLegend citation) - RRID
-
AB_2832616 (BioLegend Cat. No. 313247)
Antigen Details
- Structure
- Growth factor receptor with tyrosine kinase activity, subclass III, approximately 145 kD
- Distribution
-
Pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, acute myeloid leukemic cells (AML)
- Function
- Growth factor receptor for stem cell factor. Induces proliferation and survival of primitive hematopoietic progenitors. Potent inducer of proliferation in erythroid-committed progenitor cells. Defects in CD117 have been linked to severe anemia and a decreased number of hematopoietic progenitor cells.
- Ligand/Receptor
- c-Kit ligand
- Modification
- Multiple phosphorylation sites
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Giebel LB, et al. 1992. Oncogene 7:2207.
2. Furitsu T, et al. 1993. J. Clin. Invest. 92:1736. - Gene ID
- 3815 View all products for this Gene ID
- UniProt
- View information about CD117 on UniProt.org
Other Formats
View All CD117 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD117 (c-kit)
Human myeloid cell line TF-1 stained with purified 104D2, fo... -
PE anti-human CD117 (c-kit)
Human erythroleukemia cell line TF-1 stained with 104D2 PE 
Confocal image of human skin sample acquired using the IBEX ... -
APC anti-human CD117 (c-kit)
Human erythroleukemia cell line TF-1 stained with 104D2 APC -
Biotin anti-human CD117 (c-kit)
Human erythroleukemic cell line TF-1 stained with biotinylat... -
PE/Cyanine5 anti-human CD117 (c-kit)
Human myeloid leukemia cell line TF-1 stained with 104D2 PE/... -
PE/Cyanine7 anti-human CD117 (c-kit)
Human erythroleukemic cell line TF-1 stained with 104D2 PE/C... -
PerCP/Cyanine5.5 anti-human CD117 (c-kit)
Human erythroleukemia cell line (HEL) was stained with CD117... -
Brilliant Violet 421™ anti-human CD117 (c-kit)
Human erythroleukemia cell line (HEL) was stained with CD117... -
Brilliant Violet 605™ anti-human CD117 (c-kit)
Human erythroleukemia cell line (HEL) was stained with CD117... -
Brilliant Violet 510™ anti-human CD117 (c-kit)
Human erythroleukemia cell line (HEL) was stained with CD117... -
Brilliant Violet 650™ anti-human CD117 (c-kit)
Human erythroleukemia cell line (HEL) was stained with CD117... -
Purified anti-human CD117 (c-kit) (Maxpar® Ready)
Human TF-1a erythroleukemia cells (red) and mouse EL4 T cell... -
PE/Dazzle™ 594 anti-human CD117 (c-kit)
Human erythroleukemia cell line (HEL) was stained with CD117... -
APC/Cyanine7 anti-human CD117 (c-kit)
Human erythroleukemia cell line (HEL) was stained with CD117... -
Brilliant Violet 711™ anti-human CD117 (c-kit)
Human erythroleukemia cell line (HEL) was stained with CD117... -
FITC anti-human CD117 (c-kit)
Human erythroleukemia cell line (HEL) was stained with CD117... -
Alexa Fluor® 488 anti-human CD117 (c-kit)
Human erythroleukemia cell line (HEL) was stained with CD117... 
Confocal image of human lymph node sample acquired using the... 
Confocal image of human lymph node sample acquired using the... -
Alexa Fluor® 647 anti-human CD117 (c-kit)
Human erythroleukemia cell line (HEL) was stained with CD117... -
APC/Fire™ 750 anti-human CD117 (c-kit)
Human erythroleukemia cell line (HEL) was stained with CD117... -
Brilliant Violet 785™ anti-human CD117 (c-kit)
Human erythroleukemia cell line (HEL) was stained with CD117... -
PE anti-human CD117 (c-kit)
Typical results from human erythroleukemia cell line TF-1 st... -
APC anti-human CD117 (c-kit)
Typical results from human erythroleukemia cell line TF-1 st... -
TotalSeq™-A0061 anti-human CD117 (c-kit)
-
TotalSeq™-C0061 anti-human CD117 (c-kit)
-
TotalSeq™-B0061 anti-human CD117 (c-kit)
-
Alexa Fluor® 700 anti-human CD117 (c-kit)
Human peripheral blood mononuclear cells were stained with C... -
Spark NIR™ 685 anti-human CD117 (c-kit) Antibody
Human erythroleukemia cell line (HEL) was stained with anti-... -
APC/Fire™ 750 anti-human CD117 (c-kit)
Typical results from human erythroleukemia (HEL) cell line s... -
TotalSeq™-D0061 anti-human CD117 (c-kit)
-
PE/Cyanine7 anti-human CD117 (c-kit)
Typical results from human erythroleukemia (HEL) cell line s... -
PE/Dazzle™ 594 anti-human CD117 (c-kit)
Typical results from human erythroleukemia (HEL) cell line s... -
PerCP/Cyanine5.5 anti-human CD117 (c-kit)
Typical results from human erythroleukemia (HEL) cell line s... -
GMP APC anti-human CD117 (c-kit)
Typical results from human erythroleukemia cell line TF-1 st... -
GMP PE anti-human CD117 (c-kit)
Typical results from human erythroleukemia cell line TF-1 st... -
Spark Red™ 718 anti-human CD117 (c-kit) (Flexi-Fluor™)