PE anti-human CD25 Antibody

Pricing & Availability
Clone
BC96 (See other available formats)
Regulatory Status
RUO
Workshop
V T-072
Other Names
Low affinity IL-2R, IL-2R α chain, Tac, p55
Isotype
Mouse IgG1, κ
bc96
PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with CD25 (clone BC96) PE (solid line) or mouse IgG1, κ PE isotype control (dotted line).
  • bc96
    PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with CD25 (clone BC96) PE (solid line) or mouse IgG1, κ PE isotype control (dotted line).
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Cat # Size Price Quantity Check Availability
302605 25 tests $39.00
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302606 100 tests $94.00
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Description

CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The CD25 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications include: immunocytochemistry3.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  3. Ernst CW, et al. 2007. Clin. Exp. Immunol. 148:271. (ICC) PubMed
Product Citations
  1. Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed
  2. Alam A, et al. 2022. Cancer Cell. 40:153. PubMed
  3. Hong Y, et al. 2022. J Leukoc Biol. 112:425. PubMed
  4. Zhang H, et al. 2023. Nat Immunol. 24:96. PubMed
  5. Xu Q, et al. 2023. Nat Immunol. 24:186. PubMed
  6. Riner D, et al. 2016. PLoS Negl Trop Dis. 10:e0005180. PubMed
  7. Tang M, et al. 2019. Biomed Res Int. 2019:1050285. PubMed
  8. Miragaia RJ, et al. 2019. Immunity. 50:493. PubMed
  9. Iwahori K, et al. 2019. Sci Rep. 2.205555556. PubMed
  10. Yang M, et al. 2019. Cell Physiol Biochem. 52:1178. PubMed
  11. Ando M, et al. 2021. Cancer Res Commun. 1:41. PubMed
  12. Hu Y, et al. 2023. Front Immunol. 14:1128423. PubMed
  13. Pfister F, et al. 2023. Methods Mol Biol. 2644:331. PubMed
  14. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  15. Netsrithong R, et al. 2020. Stem Cell Research. 39:101487.. PubMed
  16. Freeman ZT, et al. 2020. J Clin Invest. 130:1405. PubMed
  17. Miao L, et al. 2020. Theranostics. 0.7625. PubMed
  18. Li X, et al. 2019. Mol Med Rep. 19:4195. PubMed
  19. Savage TM, et al. 2018. JCI Insight. 3:. PubMed
  20. Wei H, et al. 2021. Malar J. 20:89. PubMed
  21. Xun X, et al. 2021. Am J Transl Res. 13:4360. PubMed
  22. Vinogradova EV, et al. 2021. STAR Protoc. 2:100458. PubMed
  23. Klemm F, et al. 2020. Cell. 181(7):1643-1660.e17. PubMed
  24. Saadati S, et al. 2020. Avicenna J Med Biotechnol. 0.591666667. PubMed
  25. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  26. Pothlichet J, et al. 2022. Front Immunol. 13:824746. PubMed
  27. Bellini N, et al. 2022. iScience. 25:105234. PubMed
  28. Goletz C, et al. 2018. Front Immunol. 9:1614. PubMed
  29. de Boer B et al. 2018. Cancer cell. 34(4):674-689 . PubMed
  30. Lopatina T, et al. 2018. Sci Rep. 8:17458. PubMed
  31. Ramien C, et al. 2020. Cell Reports. 29(4):810-815. PubMed
  32. Kobayashi Y, et al. 2020. Int J Oncol. 999:56. PubMed
  33. James E, et al. 2016. PLoS Pathog. 12: 1005375. PubMed
  34. Chen Y, et al. 2012. PLoS One. 7:e47190. PubMed
  35. Soodgupta D, et al. 2019. Cell Rep. 29:829. PubMed
  36. Meng S, et al. 2018. Mol Med Rep. 18:4247. PubMed
  37. Zhang W, et al. 2012. J Biol Chem. 287:34157. PubMed
  38. Meléndez AV, et al. 2022. Cell Mol Life Sci. 79:513. PubMed
  39. Lee JM, et al. 2022. Lupus Sci Med. 9:. PubMed
  40. Shevyrev D, et al. 2021. Exp Ther Med. 209:21. PubMed
  41. Robertson N, et al. 2020. J Biol Chem. . PubMed
  42. Wang B, et al. 2017. Stem Cell Res Ther.. 10.1186/s13287-017-0744-6. PubMed
  43. Wu D, et al. 2020. Biomark Res. 8:3. PubMed
  44. Chen Y, et al. 2021. Transl Lung Cancer Res. 10:2193. PubMed
  45. Erdem A, et al. 2022. Nat Commun. 13:1105. PubMed
  46. You M, et al. 2021. Nat Cell Biol. 23:620. PubMed
  47. Carnevale J, et al. 2022. Nature. 609:174. PubMed
  48. Fujigaki J, et al. 2015. PLoS One. 10: 0132521. PubMed
  49. Scarneo SA, et al. 2022. Sci Rep. 12:18091. PubMed
  50. Shen X, et al. 2021. Front Immunol. 12:710750. PubMed
  51. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  52. Mengrelis K, et al. 2021. Pediatr Transplant. 25:e13930. PubMed
  53. Zhou Y, et al. 2016. J Leukoc Biol. 100: 1201 - 1211. PubMed
  54. Reichwald K, et al. 2014. PLoS One. 9:105627. PubMed
  55. Luo X, et al. 2015. J Biol Chem. 290: 28675 - 28682. PubMed
  56. Batikan O, et al. 2022. J Coll Physicians Surg Pak. 32:1004. PubMed
  57. Houtsma R, et al. 2021. STAR Protoc. 2:100864. PubMed
  58. Cohen CA, et al. 2021. Nat Commun. 12:4678. PubMed
  59. Keefe RC, et al. 2021. Sci Rep. 11:14933. PubMed
  60. Tian M, et al. 2021. Elife. 10:. PubMed
  61. Chruewkamlow N, et al. 2021. Stem Cell Res Ther. 12:520. PubMed
  62. Lima J, et al. 2016. Reprod Sci. 10.1177/1933719116653680. PubMed
  63. Imura Y, et al. 2020. JCI Insight. :5. PubMed
  64. Vinogradova EV, et al. 2020. Cell. 182(4):1009-1026.e29. PubMed
  65. Han L, et al. 2014. J Biol Chem. 289:25546. PubMed
  66. Hirschberger S, et al. 2021. EMBO Mol Med. 13:e14323. PubMed
  67. Zhang B, et al. 2020. Cell Chemical Biology. 28(1):60-69.e7. PubMed
  68. Ferraro A, et al. 2014. Proc Natl Acad Sci U S A. 111:1111. PubMed
  69. Montel‐Hagen A et al. 2019. Cell stem cell. 24(3):376-389 . PubMed
  70. Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed
  71. Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed
  72. Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed
  73. Loo Yau H, et al. 2021. STAR Protocols. 2(2):100549. PubMed
  74. Dyson MR, et al. 2020. MAbs. 12:1829335. PubMed
  75. Ajith A, et al. 2019. FASEB J. 33:5220. PubMed
  76. Wang L, et al. 2018. Oncol Lett. 15:8635. PubMed
  77. Robertson N, et al. 2020. J Biol Chem. 295:18436. PubMed
  78. Cerny D, et al. 2016. J Allergy Clin Immunol. 138:1709-1712. PubMed
  79. Fierle JK, et al. 2021. Cell Reports Medicine. 2(8):100362. PubMed
  80. Hui Z, et al. 2022. Cell Death Dis. 13:607. PubMed
  81. Högelin KA, et al. 2021. iScience. 24:103078. PubMed
  82. Choe JH, et al. 2021. Sci Transl Med. 13:. PubMed
  83. Welch JL, et al. 2020. FEBS Lett. 594:695. PubMed
  84. Wang AS, et al. 2020. Molecular Cell. 79(2):221-233.e5.. PubMed
  85. Kim N, et al. 2020. Nat Commun. 2.045138889. PubMed
RRID
AB_314275 (BioLegend Cat. No. 302605)
AB_314275 (BioLegend Cat. No. 302606)

Antigen Details

Structure
Type I transmembrane glycoprotein, 55 kD
Distribution

Activated T cells and B cells, monocytes/macrophages, Treg

Function
Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
Ligand/Receptor
IL-2
Cell Type
B cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann T. 1991. J. Biol. Chem. 266:2681.

Gene ID
3559 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org
Go To Top Version: 3    Revision Date: 12/15/2021

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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