- Clone
- W20047C (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Gp34, OX40L, CD134L, CD252, TNFSF4
- Isotype
- Rat IgG2a, κ
-
Human endothelial cells (HUVEC) were stained with anti-human CD252 (OX40L) (clone W20047C) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram)
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 389203 | 25 tests | $140.00 | |||
OX40 ligand (OX40L), also known as gp34, CD252, CD134L or TNFSF4, is a 35 kD type II transmembrane protein which belongs to TNF superfamily. The OX40 ligand is expressed on activated B cells, activated endothelial cells, mature dendritic cells, activated CTLs, activated mast cells, and airway smooth muscle cells. OX40 ligand interacts with OX40 antigen (CD134) expressed predominantly on activated T cells to regulate T cell proliferation and differentiation, and to enhance immunoglobulin secretion by activated B cells.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant human OX40L protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- RRID
-
AB_3106062 (BioLegend Cat. No. 389203)
Antigen Details
- Structure
- NGF/TNF superfamily, 35 kD
- Distribution
-
Activated B cells, activated endothelial cells, mature dendritic cells, airway smooth muscle cells
- Function
- T-cell interaction, costimulation
- Ligand/Receptor
- OX40 (CD134)
- Cell Type
- B cells, Dendritic cells, Endothelial cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
- Imura A, et al. 1996. J Exp Med. 183:2185-95.
- Ohshima Y, et al. 1997. J Immunol. 159:3838-48.
- Ito T, et al. 2005. J Exp Med. 202:1213-23.
- Ito T, et al. 2006. Proc Natl Acad.Sci. USA. 103:13138.
- Gene ID
- 7292 View all products for this Gene ID
- UniProt
- View information about CD252 on UniProt.org
Other Formats
View All CD252 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD252 (OX40L) | W20047C | FC |
| PE anti-human CD252 (OX40L) | W20047C | FC |
| TotalSeq™-A1463 anti-human CD252 (OX40L) | W20047C | PG |
| TotalSeq™-C1463 anti-human CD252 (OX40L) Antibody | W20047C | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD252 (OX40L)
Human endothelial cells (HUVEC) were stained with purified a... -
PE anti-human CD252 (OX40L)
Human endothelial cells (HUVEC) were stained with anti-human... -
TotalSeq™-A1463 anti-human CD252 (OX40L)
-
TotalSeq™-C1463 anti-human CD252 (OX40L) Antibody