PE anti-human CD254 (TRANCE, RANKL) Antibody

Pricing & Availability
Clone
MIH24 (See other available formats)
Regulatory Status
RUO
Other Names
TRANCE (TNF-related activation-induced cytokine receptor), CD254, RANK-L (receptor activator of NF-κB ligand), OPGL (Osteoprotegerin ligand), SOFA, TNFSF-11
Isotype
Mouse IgG2b, κ
A_MIH24_PE_030510
PHA-stimulated human peripheral blood mononuclear cells (3 days) stained with CD25 (clone BC96) APC and MIH24 PE
  • A_MIH24_PE_030510
    PHA-stimulated human peripheral blood mononuclear cells (3 days) stained with CD25 (clone BC96) APC and MIH24 PE
  • B_MIH24_PE_030510
    PHA-stimulated human peripheral blood mononuclear cells (3 days) stained with CD25 (clone BC96) APC and mouse IgG2b, κ PE isotype control
  • MIH24_PE_021610
    Human RANKL transfected P815 cells stained with MIH24 PE
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347503 25 tests $171.00
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347504 100 tests $341.00
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Description

Human CD254, also known as RANKL, TRANCE, and OPGL, is a type II transmembrane protein in the TNF superfamily that has two forms: a membrane-anchored protein and a secreted protein. Its extracellular domain is related to TRAIL, FasL, and TNF. CD254 is expressed on osteoblasts and activated T cells. It has been implicated in the regulation of the interactions between T cells and dendritic cells. It plays an important role in osteoblast differentiation and bone resorption. CD254 activates JNK and NF-κB and induces the expression of IL-1, IL-6, IL-12, and IL-15 in dendritic cells.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
RANKL-transfected L cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats of this clone) include: ELISA and immunohistochemical staining of paraffin-embedded tissue sections2.

Application References

(PubMed link indicates BioLegend citation)
  1. Kanamaru F, et al. 2004. Immunol. Lett. 94:239.
  2. Mueller A, et al. 2014. Arthritis Res. Ther. 16:R55. (IHC) PubMed
Product Citations
  1. Lee WS, et al. 2020. Arthritis Rheumatol. 72:1493. PubMed
  2. Meng Y,et al. 2017. Cell Death Dis. . 10.1038/cddis.2017.505. PubMed
  3. Hernndez DC, et al. 2021. Immunity. :. PubMed
  4. Liao HQ, et al. 2021. Am J Reprod Immunol. 86:e13406. PubMed
  5. Patel C, et al. 2022. Curr Protoc. 2:e400. PubMed
  6. Vanoni G, et al. 2021. eLife. 10:00. PubMed
  7. Kenty JH, et al. 2021. STAR Protocols. 2(3):100675. PubMed
RRID
AB_2256264 (BioLegend Cat. No. 347503)
AB_2256264 (BioLegend Cat. No. 347504)

Antigen Details

Structure
A TNF superfamily member.
Distribution

Expressed on osteoblasts and activated T cells.

Function
Plays an important role in osteoblast differentiation and bone resorption, regulates the interactions between T cells and dendritic cells, and T cells and B cells.
Ligand/Receptor
RANK, OPG.
Cell Type
T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokines/Chemokines
Antigen References

1. Wiethe C, et al. 2003. J. Immunol. 171:4121
2. Page G, et al. 2005. Arthritis Rheum. 52:2307
3. Ji JD, et al. 2009. J. Immunol. 183:7223

Gene ID
8600 View all products for this Gene ID
UniProt
View information about CD254 on UniProt.org
Go To Top Version: 3    Revision Date: 06/05/2020

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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