PE anti-human CD319 (CRACC) Antibody

Pricing & Availability
Clone
162.1 (See other available formats)
Regulatory Status
RUO
Other Names
CD2 like receptor activating cytotoxic cells, CD319, CS1, 19A24, SLAMF7
Isotype
Mouse IgG2b, κ
162dot1_PE_030408
Human peripheral blood lymphocytes stained with CD3 (HIT3a) APC and 162.1 PE
  • 162dot1_PE_030408
    Human peripheral blood lymphocytes stained with CD3 (HIT3a) APC and 162.1 PE
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Cat # Size Price Quantity Check Availability
331806 100 tests $292.00
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Description

CD319 is a single-pass type l transmembrane glycoprotein, expressed on NK cells, subsets of mature dendritic cells, activated B cells, and cytotoxic lymphocytes, but not in promyelocytic, B or T cell lines. Expression is highest in the spleen, lymph nodes, and peripheral blood leukocytes, and lowest in bone marrow. Additionally, it is expressed in the small intestine, stomach, appendix, lung, and trachea. CD319 is tyrosine phosphorylated in activated NK cells and is associated with 19 and 39 kD proteins. CD319 has homology with the CD2 family of receptors within the Ig superfamily. Some of the CD2 members stimulate cytotoxicity through the CD319 associated protein.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CRACC-Human IgG1 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Bouchon A, et al. 2001. J. Immunol. 167:551721.
  2. Tassi I and Colonna M. 2005. J. Immunol. 175:79968002.
  3. Veillette A. 2006. Immunol. Rev. 214:2234.
Product Citations
  1. Chroma K, et al. 2022. Cell Death Dis. 13:203. PubMed
  2. Golubovskaya V, et al. 2021. Biomedicines. 9:. PubMed
  3. Tardif V, et al. 2019. Nat Commun. 10:823. PubMed
  4. Soh KT, et al. 2021. Cytometry B Clin Cytom. 100:497. PubMed
  5. Beyer M, et al. 2012. PLoS One. 7:45466. PubMed
  6. Jenks SA et al. 2018. Immunity. 49(4):725-739 . PubMed
  7. O'Connor MH, et al. 2021. Commun Biol. 4:563. PubMed
  8. Nahi H, et al. 2022. Cell Rep Med. 3:100508. PubMed
RRID
AB_2239190 (BioLegend Cat. No. 331806)

Antigen Details

Structure
Ig superfamily, 66 kD.
Distribution

NK cells, subsets of mature dendritic cells, activated B cells, cytotoxic lymphocytes but not in promyelocytic, B or T cell lines.

Function
Regulates T cell and NK cell functions.
Cell Type
B cells, Dendritic cells, Lymphocytes, NK cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Bouchon A, et al. 2001. J. Immunol. 167:551721.
2. Tassi I and Colonna M. 2005. J. Immunol. 175:79968002.
3. Veillette A. 2006. Immunol. Rev. 214:2234.

Gene ID
57823 View all products for this Gene ID
UniProt
View information about CD319 on UniProt.org
Go To Top Version: 2    Revision Date: 06/03/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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