Purified anti-human CD319 (CRACC) Antibody

Pricing & Availability
Clone
162.1 (See other available formats)
Regulatory Status
RUO
Other Names
CD2 like receptor activating cytotoxic cells, CD319, CS1, 19A24, SLAMF7
Isotype
Mouse IgG2b, κ
162dot1_Pure_022508
Human peripheral blood lymphocytes stained with purified 162.1, followed by anti-mouse IgG FITC
  • 162dot1_Pure_022508
    Human peripheral blood lymphocytes stained with purified 162.1, followed by anti-mouse IgG FITC
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Cat # Size Price Quantity Check Availability
331802 100 µg $129.00
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Description

CD319 is a single-pass type l transmembrane glycoprotein, expressed on NK cells, subsets of mature dendritic cells, activated B cells, and cytotoxic lymphocytes, but not in promyelocytic, B or T cell lines. Expression is highest in the spleen, lymph nodes, and peripheral blood leukocytes, and lowest in bone marrow. Additionally, it is expressed in the small intestine, stomach, appendix, lung, and trachea. CD319 is tyrosine phosphorylated in activated NK cells and is associated with 19 and 39 kD proteins. CD319 has homology with the CD2 family of receptors within the Ig superfamily. Some of the CD2 members stimulate cytotoxicity through the CD319 associated protein.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CRACC-Human IgG1 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Bouchon A, et al. 2001. J. Immunol. 167:551721.
  2. Tassi I and Colonna M. 2005. J. Immunol. 175:79968002.
  3. Veillette A. 2006. Immunol. Rev. 214:2234.
Product Citations
  1. Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
  2. Quentmeier H, et al. 2019. Sci Rep. 9:8218. PubMed
  3. Chakhtoura M, et al. 2021. PLoS Pathog. 17:e1009732. PubMed
RRID
AB_961330 (BioLegend Cat. No. 331802)

Antigen Details

Structure
Ig superfamily, 66 kD.
Distribution

NK cells, subsets of mature dendritic cells, activated B cells, cytotoxic lymphocytes but not in promyelocytic, B or T cell lines.

Function
Regulates T cell and NK cell functions.
Cell Type
B cells, Dendritic cells, Lymphocytes, NK cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Bouchon A, et al. 2001. J. Immunol. 167:551721.
2. Tassi I and Colonna M. 2005. J. Immunol. 175:79968002.
3. Veillette A. 2006. Immunol. Rev. 214:2234.

Gene ID
57823 View all products for this Gene ID
UniProt
View information about CD319 on UniProt.org
Go To Top Version: 3    Revision Date: 08/12/2022

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