PE anti-human CD45RB Antibody

Pricing & Availability
Clone
MEM-55 (See other available formats)
Regulatory Status
RUO
Workshop
V CD45.08
Other Names
CD45RB
Isotype
Mouse IgG2b, κ
MEM-55
Human peripheral blood lymphocytes stained with MEM-55 PE
  • MEM-55
    Human peripheral blood lymphocytes stained with MEM-55 PE
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Cat # Size Price Quantity Check Availability
310204 100 tests $268.00
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Description

CD45RB is a 180-240 kD single chain type I membrane glycoprotein. It is an exon 5 splice variant of the tyrosine phosphatase CD45. The CD45RB isoform is expressed on thymocytes, T cell subsets, B cells, monocytes/macrophages, granulocytes, and dendritic cells. CD45RB enhances both T cell receptor and B cell receptor signaling mediated activation. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines. The MEM-55 antibody recognizes a silaidase-sensitive epitope of CD45.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human thymocytes and T lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The MEM-55 antibody is specific for a glycosylated-CD45RB epitope that is highly expressed on memory B cells and plasmablasts but not on naïve B cells. Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting, and immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Horejsi V, et al. 1988. Folia Biol. 34:23.
  2. Bazil V, et al. 1989. Immunogenetics 29:202.
  3. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  4. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  5. Koethe S, et al. 2011. J. Leukoc. Biol. 90:5.
Product Citations
  1. Glass MC, et al. 2022. Cell Rep. 39:110728. PubMed
  2. Krummey SM, et al. 2020. Cell Reports. 30(5):1282-1291.e5.. PubMed
  3. Yuan J et al. 2018. Molecular cell. 72(2):380-394 . PubMed
  4. Szodoray P, et al. 2021. Cell Reports. 36(6):109525. PubMed
RRID
AB_314807 (BioLegend Cat. No. 310204)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane, 180-240 kD (exon 5 splicing of CD45 gene)
Distribution

Thymocytes, T cell subset, B cells, monocytes/macrophages, granulocytes, dendritic cells

Function
Enhances TCR and BCR signaling
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.

Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45RB on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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