PE anti-human FOXP3 Antibody

Pricing & Availability
Clone
259D (See other available formats)
Regulatory Status
RUO
Other Names
Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
Isotype
Mouse IgG1, κ
259D_PE_FOXP3_Antibody_ICFC_1_041515
Human peripheral blood lymphocytes were surface stained with CD4 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with FOXP3 (clone 259D) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • 259D_PE_FOXP3_Antibody_ICFC_1_041515
    Human peripheral blood lymphocytes were surface stained with CD4 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with FOXP3 (clone 259D) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • 259D_PE_FOXP3_Antibody_ICFC_2_041515
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320207 25 tests $183.00
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320208 100 tests $377.00
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Description

FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 δ2) lacking exon 2. The 259D antibody recognizes human FOXP3 epitope in the region of amino acids 105-235.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus, Rhesus, Baboon, Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length FOXP3 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1, and immunohistochemical staining1 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. The 259D antibody gives strong positivity on paraffin and frozen sections and the antibody stains some epithelial cells. The binding of 206D to FOXP3 can be partially blocked by 259D, but 206D does not show significant blocking effect on 259D binding.

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Roncador G, et al. 2005 Eur. J. Immunol. 35:1681.
  2. Yang ZZ, et al. 2006. Blood 107:3639. PubMed
  3. Gavin MA, et al. 2006. P. Natl. Acad. Sci. USA 103:6659. PubMed
  4. Groh V, et al. 2006. Nature Immunology 7:755. PubMed
  5. Tran DQ, et al. 2007. Blood doi:10.1182/blood-2007-06-094656.PubMEd
  6. Long SA, et al. 2008. J Autoimmun. 30:293. PubMed
  7. Gong G, et al. 2009. Blood 113:837. PubMed
  8. Long SA, et al. 2009. Eur J. Immunol. 39:612. PubMed
  9. Long SA, et al. 2010. Diabetes. 59:407. PubMed
  10. Ferraro A, et al. 2014. PNAS. 111:1111. PubMed
  11. Vudattu NK, et al. 2014. J Immunol. 193:587. PubMed
  12. Dupont G, et al. 2014. Cytokine. 69:146. PubMed
Product Citations
  1. Gan X, et al. 2022. Proc Natl Acad Sci U S A. 119:e2200879119. PubMed
  2. Ariafar A, et al. 2020. Heliyon. 6:e05556. PubMed
  3. Krebs FK, et al. 2021. Cancer Med. . PubMed
  4. Zimmer N, et al. 2020. Cancers (Basel). 12:00. PubMed
  5. Eberhardt K, et al. 2015. Clin Infect Dis. 61: 1615 - 1623. PubMed
  6. Woolsey C, et al. 2020. Scientific Reports. 10(1):3071.. PubMed
  7. Burbano YCB, et al. 2019. Rev Soc Bras Med Trop. 52:e20190101. PubMed
  8. Park K, et al. 2008. Cancer Res. 68:8400. PubMed
  9. Weiss E, et al. 2011. J Immunol. 187:1684. PubMed
  10. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  11. Popmihajlov Z, Smith K 2008. PLoS One. 1.222916667. PubMed
  12. Voo K, et al. 2009. Proc Natl Acad Sci U S A. 106:4793. PubMed
  13. Cheng J, et al. 2022. iScience. 25:103588. PubMed
  14. Garg G, et al. 2012. J Immunol. 188:4644. PubMed
  15. Pierini S, et al. 2020. JCI Insight. 5:00. PubMed
  16. Voo K, et al. 2013. J Immunol. 191:3641. PubMed
  17. Kuranda K, et al. 2018. J Clin Invest. 128:5267. PubMed
RRID
AB_492982 (BioLegend Cat. No. 320207)
AB_492982 (BioLegend Cat. No. 320208)

Antigen Details

Structure
Forkhead/winged-helix transcription factor family, approximately 50 kD, contains zinc finger and forkhead domains
Distribution

Nuclear; expressed in T regulatory cells

Function
Transcription factor proposed to be a master regulatory gene in T regulatory cell development and a critical factor for immune homeostasis
Interaction
Interacts with DNA
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Hori S, et al. 2003. Science 299:1057.

Regulation
FOXP3 is present at high levels in T regulatory cell can also be induced by T cell activation
Gene ID
50943 View all products for this Gene ID
UniProt
View information about FOXP3 on UniProt.org
Go To Top Version: 7    Revision Date: 06/04/2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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