Purified anti-human FOXP3 Antibody

Pricing & Availability
Clone
259D (See other available formats)
Regulatory Status
RUO
Other Names
Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
Isotype
Mouse IgG1, κ
A_259D_110105
Cell extract from HEK293T cells transfected with human FoxP3 cDNA was resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-FoxP3 antibody (clone 259D). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
  • A_259D_110105
    Cell extract from HEK293T cells transfected with human FoxP3 cDNA was resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-FoxP3 antibody (clone 259D). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
  • B_FOXP3-259D-IHC_paraffin
    Formalin-fixed, paraffin-embedded Cynomolgus kidney was treated with EDTA pH 8.0 using a high pressure cooker prior to staining. Staining was carried out with monoclonal anti-FoxP3 (clone 259D) at 10 µg/ml followed by biotinylated goat anti-mouse and streptavidin HRP. Staining was visualized with DAB substrate and the tissue counterstained with hematoxylin.
  • C_259D_PURE_FOXP3_Antibody_SB_111423
    SeqIF™ (sequential immunofluorescence) staining on COMET™ of Purified anti-FOXP3 (clone 259D, yellow) on formalin-fixed paraffin-embedded human head and neck squamous cell carcinoma (HNSCC) tissue at 10 µg/mL. Alexa Fluor™ Plus 647 Goat anti-Mouse IgG antibody (Lunaphore, Cat. No. DR647MS) was used as a secondary antibody. Nuclei were counterstained with DAPI (blue). Tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing.
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Cat # Size Price Quantity Check Availability
320201 25 µg $129.00
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320202 100 µg $265.00
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Description

FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 δ2) lacking exon 2. The 259D antibody recognizes human FOXP3 epitope in the region of amino acids 105-235.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus, Rhesus, Baboon, Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length FOXP3 protein
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
ICFC - Reported in the literature, not verified in house
SB - Community verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. For Western blotting, the suggested working dilution(s) is ≤ 5.0 µg/ml in antibody dilution buffer. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1, and immunohistochemical staining1 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. The 259D antibody gives strong positivity on paraffin and frozen sections and the antibody stains some epithelial cells. The binding of 206D to FOXP3 can be partially blocked by 259D, but 206D does not show significant blocking effect on 259D binding.

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Additional Product Notes

For the use of this antibody in spatial biology applications, we have partnered with Lunaphore Technologies for demonstration of our antibodies on the COMET™. The COMET™ platform is an automated, end-to-end spatial biology solution developed for rapid and flexible multiplex tissue profiling. More information on the COMET™ and a complete list of our antibodies that have been demonstrated on the COMET™ can be found here.

Application References

(PubMed link indicates BioLegend citation)
  1. Roncador G, et al. 2005 Eur. J. Immunol. 35:1681.
  2. Yang ZZ, et al. 2006. Blood 107:3639. PubMed
  3. Gavin MA, et al. 2006. P. Natl. Acad. Sci. USA 103:6659. PubMed
  4. Groh V, et al. 2006. Nature Immunology 7:755. PubMed
  5. Tran DQ, et al. 2007. Blood doi:10.1182/blood-2007-06-094656.PubMEd
  6. Long SA, et al. 2008. J Autoimmun. 30:293. PubMed
  7. Gong G, et al. 2009. Blood 113:837. PubMed
  8. Long SA, et al. 2009. Eur J. Immunol. 39:612. PubMed
  9. Long SA, et al. 2010. Diabetes. 59:407. PubMed
  10. Ferraro A, et al. 2014. PNAS. 111:1111. PubMed
  11. Vudattu NK, et al. 2014. J Immunol. 193:587. PubMed
  12. Dupont G, et al. 2014. Cytokine. 69:146. PubMed
Product Citations
  1. Zhang S, et al. 2023. Front Cell Infect Microbiol. 13:1165790. PubMed
  2. Passerini L, et al. 2008. Int Immunol. 1.125694444. PubMed
  3. Wang R, et al. 2009. Proc Natl Acad Sci U S A. 106:13439. PubMed
  4. Humblet-Baron S, et al. 2007. J Clin Invest. 117:407. PubMed
  5. Karandikar V 2008. Blood. 111:463. PubMed
  6. Cheng B, et al. 2022. Cancer Commun (Lond). 42:17. PubMed
  7. Kaffes I, et al. 2019. Oncoimmunology. 8:e1655360. PubMed
  8. Kegler A, et al. 2019. Oncoimmunology. 8:e1621676. PubMed
  9. Pan C, et al. 2021. Oncoimmunology. 10:1883890. PubMed
RRID
AB_430884 (BioLegend Cat. No. 320201)
AB_430884 (BioLegend Cat. No. 320202)

Antigen Details

Structure
Forkhead/winged-helix transcription factor family, approximately 50 kD, contains zinc finger and forkhead domains
Distribution

Nuclear; expressed in T regulatory cells

Function
Transcription factor proposed to be a master regulatory gene in T regulatory cell development and a critical factor for immune homeostasis
Interaction
Interacts with DNA
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Hori S, et al. 2003. Science 299:1057.

Regulation
FOXP3 is present at high levels in T regulatory cell can also be induced by T cell activation
Gene ID
50943 View all products for this Gene ID
UniProt
View information about FOXP3 on UniProt.org
Go To Top Version: 7    Revision Date: 11/14/2023

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