PE anti-human FOXP3 Antibody

Pricing & Availability
Clone
206D (See other available formats)
Regulatory Status
RUO
Other Names
Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
Isotype
Mouse IgG1, κ
206D_PE_FOXP3_Antibody_ICFC_1_041515
Human peripheral blood lymphocytes were surface stained with CD4 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with FOXP3 (clone 206D) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • 206D_PE_FOXP3_Antibody_ICFC_1_041515
    Human peripheral blood lymphocytes were surface stained with CD4 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with FOXP3 (clone 206D) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • 206D_PE_FOXP3_Antibody_ICFC_2_041515
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Cat # Size Price Quantity Check Availability
320107 25 tests $183.00
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320108 100 tests $377.00
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Description

FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 δ2) lacking exon 2. The 206D antibody recognizes human FOXP3 epitope in the region of amino acids 105-235.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon, Cynomolgus, Rhesus, Pigtailed Macaque
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length FOXP3 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The FOXP3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1 and formalin-fixed paraffin-embedded sections1,8,19-20, and Western blotting1. The binding of 206D to FOXP3 can be partially blocked by 259D, but 206D does not show significant blocking effect on 259D binding.

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Roncador G, et al. 2005. Eur. J. Immunol. 35:1681.(IHC)
  2. Yang ZZ, et al. 2006. Blood 107:3639.
  3. Liu W, et al. 2006. J. Exp. Med. 203:1701.PubMed
  4. Bollyky PL, et al. 2007. J. Immunol. 179:744.
  5. Bell MP, et al. 2007. J. Immunol. 179:1893.
  6. Tran DQ, et al. 2007. Blood doi:10.1182/blood-2007-06-094656. PubMed
  7. Gao Q,et al.2007.J Clin Oncol.25:2586.(IHC) PubMed
  8. Pillai V,et al. 2008. Blood 111:463. PubMed
  9. Zheng Y, et al. 2008. J. Immunol. 181:1683. PubMed
  10. Zonios DI, et al. 2008.Blood112:287. PubMed
  11. Kavanagh B, et al. 2008. Blood. PubMed
  12. Nevala WK, et al. 2009. Clin Cancer Res. 15:1931. PubMed
  13. Grant J, et al. 2009. Cytometry B Clin Cytom. 76:69. PubMed
  14. Nigam P, et al. 2010. J. Immunol. 184:1690. PubMed
  15. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (ICFC) PubMed
  16. Hartigan-O'Connor DJ,et al.2007.J Exp Med.204:2679. PubMed
  17. Raghaven S, et al. 2009. Ann Rheum Dis. 68:1908. PubMed
  18. Hodi FS, et al. 2014. Cancer Immunol Res. 2:632.(IHC) PubMed
  19. Sziros E, et al. 2015. Clin Cancer Res. 21:2840.(IHC) PubMed
Product Citations
  1. Donadei C, et al. 2023. J Clin Med. 12:. PubMed
  2. Imai H, et al. 2023. iScience. 26:106822. PubMed
  3. Côrte-Real BF, et al. 2022. Front Immunol. 13:1005965. PubMed
  4. Zhang B, et al. 2023. Signal Transduct Target Ther. 8:28. PubMed
  5. Cao B, et al. 2022. Nat Commun. 13:6203. PubMed
  6. Fuhrman C, et al. 2015. J Immunol. 195: 145 - 155. PubMed
  7. Ansari A, et al. 2007. J Autoimmun. 28:152. PubMed
  8. Zeng W, et al. 2017. Front Immunol. 0.806944444. PubMed
  9. Marques RM, et al. 2021. Cell Death Differ. 28:3140. PubMed
  10. Koskimaa H, et al. 2017. Eur J Clin Microbiol Infect Dis. 10.1007/s10096-017-2958-z. PubMed
  11. Maier L, et al. 2009. J Immunol. 182:1541. PubMed
  12. Gupta R, et al. 2022. Front Immunol. 13:886442. PubMed
  13. Chen M, et al. 2021. Cancers (Basel). 13:. PubMed
  14. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  15. Porsche CE, et al. 2021. JCI Insight. 6:. PubMed
  16. Gorczynski RM, et al. 2017. Immunology. 150:418. PubMed
  17. Dean JW, et al. 2020. J Autoimmun. 108:102417. PubMed
  18. Dang Y, et al. 2007. Clin Cancer Res . 1.932638889. PubMed
  19. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  20. Shevyrev D, et al. 2021. Exp Ther Med. 209:21. PubMed
  21. Longhi M, et al. 2014. PLoS One. 9:87956. PubMed
  22. Luo Y, et al. 2021. Nat Commun. 12:3913. PubMed
  23. Parsons E, et al. 2016. PLoS One. 11:e0167841. PubMed
  24. Van Zeebroeck L, et al. 2021. Front Immunol. 12:655122. PubMed
  25. OConnor RA, et al. 2021. OncoImmunology. 10(1):1940675. PubMed
  26. Platten M, et al. 2021. Nature. 592:463. PubMed
  27. Subudhi SK, et al. 2021. J Immunother Cancer. 9:. PubMed
  28. Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
  29. Sun J, et al. 2016. Sci Rep. 6:35855. PubMed
  30. Wang T et al. 2018. Immunity. 49(3):504-514 . PubMed
  31. Lai Y, et al. 2022. Clin Transl Med. 12:e999. PubMed
  32. Weinberg A, et al. 2015. PLoS One. 10:122431. PubMed
  33. Zhai Y, et al. 2018. Autophagy. 1.714583333. PubMed
  34. Zonios D, et al. 2008. Blood. 112:287. PubMed
  35. Wang H, et al. 2019. Stem Cells Int. 2019:4686132. PubMed
  36. Dhandapani H, et al. 2021. J Gynecol Oncol. 32:e59. PubMed
RRID
AB_492986 (BioLegend Cat. No. 320107)
AB_492986 (BioLegend Cat. No. 320108)

Antigen Details

Structure
Forkhead/winged-helix transcription factor family, approximately 50 kD, contains zinc finger and forkhead domains
Distribution

Nuclear; expressed in T regulatory cells

Function
Transcription factor proposed to be a master regulatory gene in T regulatory cell development and a critical factor for immune homeostasis
Interaction
Interacts with DNA
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Hori S, et al. 2003. Science 299:1057.
2. Gandhi R, et al. 2010. Nat. Immunol. 11:846.

Regulation
FOXP3 is present at high levels in T regulatory cells, it can also be induced by T cell activation.
Gene ID
50943 View all products for this Gene ID
UniProt
View information about FOXP3 on UniProt.org
Go To Top Version: 6    Revision Date: 12/16/2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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