PE anti-human IL-9 Antibody

Pricing & Availability
Clone
MH9A4 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-9, P40, Mast cell growth factor (MCGF), Megakaryoblast growth factor, T cell growth factor-3 (TCGF-3)
Isotype
Mouse IgG2b, κ
MH94A_PE_081810
Enriched human CD4+ T cells were stimulated with PMA+ionomycin, then intracellular stained with anti-IFN-γ (4S.B3) FITC and MH9A4 PE
  • MH94A_PE_081810
    Enriched human CD4+ T cells were stimulated with PMA+ionomycin, then intracellular stained with anti-IFN-γ (4S.B3) FITC and MH9A4 PE
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Cat # Size Price Quantity Check Availability
507604 25 tests $117.00
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507603 50 µg $183.00
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507605 100 tests $304.00
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Description

IL-9 is a potent, T cell-derived, T cell growth factor which can also enhance mast cell activity and IL-3- or IL-4- dependent proliferation of bone marrow-derived mast cells. IL-9 synergizes with erythropoietin to promote erythroid colony formation. IL-9 has also been reported to protect human T cells from apoptosis induced by IL-2 withdrawal. IL-9 is upregulated in human eosinophils by TNF-α and IL1-β. IL-9 has been reported to downregulate the oxidative burst in activated human alveolar macropahges and induce TGF-β production. The MH9A4 antibody reacts with human IL-9. The MH9A4 antibody can neutralize the bioactivity of natural or recombinant IL-9.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Baculovirus-expressed, recombinant human IL-9
Formulation
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

ELISA Capture2: The purified MH9A4 antibody is useful as the capture antibody in a human IL-9 sandwich ELISA assay, when used in conjunction with the biotinylated MH9D1 antibody as the detecting antibody.
For human IL-9 neutralization assay, the LEAF™ purified MH9D1 antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of human IL-9 bioactivity.

Application References

(PubMed link indicates BioLegend citation)
  1. Jenmalm M, et al. 2001. Clin. Exptl. Aller. 31:1528.
  2. Faulkner H, et al. 2002. J. Infec. Diseas. 185:665.
  3. Chen J, et al. 2008. Blood 111:5163. PubMed
  4. Chang HC, et al. 2010. Nat. Immunol. 11:527. (ELISA) PubMed
  5. Lozano E, et al. 2012. J Immunol. 188:3869. PubMed.
Product Citations
  1. Xue G, et al. 2021. Cancer Cell. 39:1610. PubMed
  2. Golebski K, et al. 2019. Nat Commun. 10:2162. PubMed
  3. Schwartz DM et al. 2019. Immunity. 50(1):106-120 . PubMed
  4. Gomez-Rodriguez J, et al. 2016. Nat Commun. 7:10857. PubMed
  5. Tomalka J, Jesus T, Ramakrishnan P 2016. J Leukoc Biol. 100: 111 - 120. PubMed
  6. Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed
  7. Roy S, et al. 2021. Nat Commun. 12:3182. PubMed
  8. Sparber F, et al. 2019. Cell Host Microbe. 25:389. PubMed
  9. Tian T, et al. 2015. PLoS One. 10: 0131761. PubMed
RRID
AB_315485 (BioLegend Cat. No. 507604)
AB_315485 (BioLegend Cat. No. 507603)
AB_315485 (BioLegend Cat. No. 507605)

Antigen Details

Structure
Cytokine; 32-39 kD (Mammalian)
Bioactivity
Potentiates production of IgG, IgM, and IgE by IL-4-induced B lymphocytes; regulates granzyme family proteases; enhances proliferation of bone marrow mast cells with IL-3; production of IL-6 by mast cells
Cell Sources
IL-2 activated TH2 lymphocytes, Hodgkin's lymphoma
Cell Targets
T lymphocytes, erythroid precursors
Receptors
IL-9R
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Quesniaux V. 1992. Research Immunology 143:385.
3. Renauld J, et al. 1993. Adv. Immunol. 54:79.
4. Yang Y. 1992. Leuk. Lymphoma 8:441.

Regulation
Upregulated by IL-1 after TCR activation, TNF-α; downregulated by antibodies to IL-2
Gene ID
3578 View all products for this Gene ID
UniProt
View information about IL-9 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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