- Clone
- QA20A25 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Latency Associated Peptide (LAP), Transforming growth factor beta 1 (TGF-b1), TGFB1, DPD1, TGF-beta1
- Isotype
- Mouse IgG1, κ
-
Human peripheral blood mononuclear cells were stimulated with PHA for 2 days. Cells were then stained with anti-human CD4 APC and anti-human LAP recombinant (clone QA20A25) PE (left) or mouse IgG1, κ PE (right).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 364403 | 25 tests | $146.00 | |||
| 364404 | 100 tests | $360.00 | |||
TGF-β1 (transforming growth factor β1) is a multifunctional cytokine which regulates cellular proliferation and differentiation. It is ubiquitously expressed by many types of cells. Platelets express high level of TGF-β. TGF-β is synthesized as a large protein precursor and then secreted as a complex of TGF-β and LAP (latency-associated peptide), in which LAP noncovalently associates with the dimeric mature TGF-β to prevent its activity. TGF-β requires activation before it binds to its receptors and exerts functions. It has been reported that LAP-TGF-β binds to the integrins αvβ1, αvβ6, αvβ8, and α8β1 through RGD domain. TGF-β plays important roles in control proliferation and differentiation of epithelial cells, endothelial cells, fibroblasts, neurons, osteoclasts, and osteoblasts. TGF-β is believed to be important in the regulation of the development of Treg, Th17, and Th9 cells. A recent study has shown that LAP is an activated Treg surface marker.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Product Citations
-
- RRID
-
AB_2910407 (BioLegend Cat. No. 364403)
AB_2910407 (BioLegend Cat. No. 364404)
Antigen Details
- Structure
- About 400 amino acids, N-terminal signal peptide is required for secretion from a cell, a pro-region (LAP) and C-terminal region that becomes the mature TGF-β molecule following its release from the pro-region by proteolytic cleavage.
- Distribution
-
Ubiquitously expressed by many kinds of cells
- Function
- Multifunctional cytokine, regulates cells proliferation, and differentiation
- Ligand/Receptor
- TGF-βRI, -RII, -RIII, CD105, LTBP, integrins
- Antigen References
-
- Yi JJ, et al. 2010. Cell 142:144.
- Tran DQ, et al. 2009. Blood 113:5125.
- Lu M, et al. 2002. J. Cell. Sci. 115:4641.
- Khalil N. 1999. Microbes Infect. 1:1255.
- Gene ID
- 7040 View all products for this Gene ID
- UniProt
- View information about LAP on UniProt.org
Other Formats
View All LAP Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human LAP Recombinant Antibody | QA20A25 | FC |
| PE anti-human LAP Recombinant Antibody | QA20A25 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-human LAP Recombinant Antibody
Human LAP transfected cells were stained with purified anti-... -
PE anti-human LAP Recombinant Antibody
Human peripheral blood mononuclear cells were stimulated wit...