- Clone
- QA20A25 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Latency Associated Peptide (LAP), Transforming growth factor beta 1 (TGF-b1), TGFB1, DPD1, TGF-beta1
- Isotype
- Mouse IgG1, κ
-
Human LAP transfected cells were stained with purified anti-human LAP (TGF-β1) (clone QA20A25) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram) followed by anti-mouse IgG biotin and Streptavidin-APC.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 364402 | 100 µg | $194.00 | |||
TGF-β1 (transforming growth factor β1) is a multifunctional cytokine which regulates cellular proliferation and differentiation. It is ubiquitously expressed by many types of cells. Platelets express high level of TGF-β. TGF-β is synthesized as a large protein precursor and then secreted as a complex of TGF-β and LAP (latency-associated peptide), in which LAP noncovalently associates with the dimeric mature TGF-β to prevent its activity. TGF-β requires activation before it binds to its receptors and exerts functions. It has been reported that LAP-TGF-β binds to the integrins αvβ1, αvβ6, αvβ8, and α8β1 through RGD domain. TGF-β plays important roles in control proliferation and differentiation of epithelial cells, endothelial cells, fibroblasts, neurons, osteoclasts, and osteoblasts. TGF-β is believed to be important in the regulation of the development of Treg, Th17, and Th9 cells. A recent study has shown that LAP is an activated Treg surface marker.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2910406 (BioLegend Cat. No. 364402)
Antigen Details
- Structure
- About 400 amino acids, N-terminal signal peptide is required for secretion from a cell, a pro-region (LAP) and C-terminal region that becomes the mature TGF-β molecule following its release from the pro-region by proteolytic cleavage.
- Distribution
-
Ubiquitously expressed by many kinds of cells
- Function
- Multifunctional cytokine, regulates cells proliferation, and differentiation
- Ligand/Receptor
- TGF-βRI, -RII, -RIII, CD105, LTBP, integrins
- Cell Type
- B cells, Dendritic cells, T cells, Tregs
- Biology Area
- Adaptive Immunity, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Inhibitory Molecules, Neuroinflammation, Neuroscience, Signal Transduction
- Molecular Family
- Cytokine/Chemokine Receptors, Growth Factors
- Antigen References
-
- Yi JJ, et al. 2010. Cell 142:144.
- Tran DQ, et al. 2009. Blood 113:5125.
- Lu M, et al. 2002. J. Cell. Sci. 115:4641.
- Khalil N. 1999. Microbes Infect. 1:1255.
- Gene ID
- 7040 View all products for this Gene ID
- UniProt
- View information about LAP on UniProt.org
Other Formats
View All LAP Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human LAP Recombinant Antibody | QA20A25 | FC |
| PE anti-human LAP Recombinant Antibody | QA20A25 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human LAP Recombinant Antibody
Human LAP transfected cells were stained with purified anti-... -
PE anti-human LAP Recombinant Antibody
Human peripheral blood mononuclear cells were stimulated wit...