PE anti-mouse CD117 (c-Kit) Antibody

Pricing & Availability
Clone
2B8 (See other available formats)
Regulatory Status
RUO
Other Names
c-KIT, Stem Cell Factor Receptor (SCFR)
Isotype
Rat IgG2b, κ
2B8_111105
C57BL/6 bone marrow cells stained with 2B8 PE and CD11b FITC
  • 2B8_111105
    C57BL/6 bone marrow cells stained with 2B8 PE and CD11b FITC
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability
105807 50 µg $98.00
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105808 200 µg $252.00
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Description

CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse bone marrow mast cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.

Application References

(PubMed link indicates BioLegend citation)
  1. Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC)
  2. Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC)
  3. Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC)
  4. Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC)
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Chung Y, et al. 2014. J Vis Exp. 89: 51660. PubMed
  2. Casellas Roman HL, et al. 2020. Leuk Res. 94:106372. PubMed
  3. Sandovici I, et al. 2022. Dev Cell. 57:63. PubMed
  4. Kropp L, et al. 2020. Clin Exp Allergy. 50:213. PubMed
  5. Cela D, et al. 2022. J Leukoc Biol. 111:1235. PubMed
  6. Alam A, et al. 2022. Cancer Cell. 40:153. PubMed
  7. Roy IM, et al. 2022. iScience. 25:105171. PubMed
  8. Chen Y, et al. 2023. Stem Cells Int. 2023:7482546. PubMed
  9. Sohn J, et al. 2022. Blood Adv. 6:2557. PubMed
  10. Li X, et al. 2022. Cell. 185:1709. PubMed
  11. Cacialli P, et al. 2022. EMBO J. 41:e108536. PubMed
  12. Schönberger K, et al. 2023. Anal Chem. 95:4325. PubMed
  13. Boers R, et al. 2023. Nat Biotechnol. . PubMed
  14. Vanneste D, et al. 2023. Nat Immunol. 24:827. PubMed
  15. Capellmann S, et al. 2023. Front Immunol. 14:1154416. PubMed
  16. van Os BW, et al. 2023. Front Cardiovasc Med. 10:1171764. PubMed
  17. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  18. Groenen AG, et al. 2022. J Lipid Res. 63:100167. PubMed
  19. Sharma GP, et al. 2021. PLoS One. 16:e0259042. PubMed
  20. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  21. Mitroulis I et al. 2018. Cell. 172(1-2):147-161 . PubMed
  22. Nguyen N, et al. 2022. iScience. 25:103679. PubMed
  23. Kakiuchi K, et al. 2014. Biol Reprod. 90:82. PubMed
  24. Sykes DB et al. 2016. Cell. 167(1):171-186 . PubMed
  25. Schmitt M et al. 2018. Cell reports. 24(9):2312-2328 . PubMed
  26. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  27. Tamura M, et al. 2019. Sci Rep. 9:8171. PubMed
  28. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  29. Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed
  30. Svahn S, et al. 2015. Infect Immun . 83:514. PubMed
  31. Klimovich B, et al. 2022. J Exp Clin Cancer Res. 41:80. PubMed
  32. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  33. Kawakami R, et al. 2021. Immunity. 54(5):947-961.e8. PubMed
  34. Nakano N, et al. 2011. J Biol Chem. 286:12042. PubMed
  35. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  36. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  37. Liu T, et al. 2022. Front Immunol. 13:901349. PubMed
  38. Cho K, et al. 2021. iScience. 24:103117. PubMed
  39. Qian J, et al. 2021. Int J Mol Sci. 22:. PubMed
  40. Yang T, et al. 2022. Nat Commun. 13:4464. PubMed
  41. Ding P, et al. 2022. Bone Res. 10:42. PubMed
  42. Zhou J, et al. 2019. Nat Commun. 10:2427. PubMed
  43. Svahn S, et al. 2016. Infect Immun . 84: 1205-1213. PubMed
  44. Pervolarakis N, et al. 2020. Cell Rep. 108273:33. PubMed
  45. Quick M, et al. 2012. Am J Physiol Regul Integr Comp Physiol. 303:580. PubMed
  46. Bennett FC, et al. 2018. Neuron. 98:1170. PubMed
  47. Schönberger K, et al. 2022. STAR Protoc. 3:101408. PubMed
  48. Grainger N, et al. 2020. J Physiol. 598:3283. PubMed
  49. Shu Z, et al. 2020. Mar Drugs. 18:00. PubMed
  50. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  51. Asada S, et al. 2018. Nat Commun. 9:2733. PubMed
  52. Sackstein C 2014. Proc Natl Acad Sci U S A. 111:10696. PubMed
  53. Yamazaki S, et al. 2015. J Immunol. 195: 3427 - 3435. PubMed
  54. Yoshinaga M, et al. 2022. Nat Commun. 13:6435. PubMed
  55. Ghazaryan S, et al. 2014. Mol Cell Biol. 34:2833. PubMed
  56. Zhong C, et al. 2020. Immunity. 52(1):83-95.e4.. PubMed
  57. Sakashita A, et al. 2019. Biol Reprod. 100:697. PubMed
  58. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
  59. Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed
  60. Wang C, et al. 2008. Am J Physiol Regul Integr Comp Physiol. 294:R811. PubMed
  61. Takayama G, et al. 2013. Int Immunol. 25:215. PubMed
  62. Roy IM, et al. 2021. Bio Protoc. 11:e4130. PubMed
  63. Zhang J, et al. 2021. Stem Cell Res Ther. 12:579. PubMed
  64. Liu Q, et al. 2016. Cell Death Dis. 1.93125. PubMed
  65. Johnson JL et al. 2018. Immunity. 48(2):243-257 . PubMed
RRID
AB_313216 (BioLegend Cat. No. 105807)
AB_313216 (BioLegend Cat. No. 105808)

Antigen Details

Structure
Ig superfamily, 145 kD
Distribution

Hematopoietic stem cells, AML, mast cells

Function
Growth factor receptor, tyrosine kinase
Ligand/Receptor
Stem Cell Factor (SCF)
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5.

Gene ID
16590 View all products for this Gene ID
UniProt
View information about CD117 on UniProt.org

Other Formats

View All CD117 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD117 (c-Kit) 2B8 FC
Biotin anti-mouse CD117 (c-Kit) 2B8 FC
FITC anti-mouse CD117 (c-Kit) 2B8 FC
PE anti-mouse CD117 (c-Kit) 2B8 FC
PE/Cyanine5 anti-mouse CD117 (c-Kit) 2B8 FC
Purified anti-mouse CD117 (c-Kit) 2B8 FC,IHC-F
PE/Cyanine7 anti-mouse CD117 (c-Kit) 2B8 FC
Alexa Fluor® 488 anti-mouse CD117 (c-Kit) 2B8 FC,IHC-F
Alexa Fluor® 647 anti-mouse CD117 (c-Kit) 2B8 FC,IHC-F
Pacific Blue™ anti-mouse CD117 (c-Kit) 2B8 FC
PerCP/Cyanine5.5 anti-mouse CD117 (c-kit) 2B8 FC
PerCP anti-mouse CD117 (c-kit) 2B8 FC
APC/Cyanine7 anti-mouse CD117 (c-kit) 2B8 FC
Brilliant Violet 421™ anti-mouse CD117 (c-Kit) 2B8 FC,SB
Purified anti-mouse CD117 (c-Kit) (Maxpar® Ready) 2B8 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD117 (c-Kit) 2B8 FC
Brilliant Violet 711™ anti-mouse CD117 (c-Kit) 2B8 FC
Alexa Fluor® 594 anti-mouse CD117 (c-Kit) 2B8 IHC-F,3D IHC
APC/Fire™ 750 anti-mouse CD117 (c-Kit) 2B8 FC
Brilliant Violet 510™ anti-mouse CD117 (c-Kit) 2B8 FC
Brilliant Violet 785™ anti-mouse CD117 (c-Kit) 2B8 FC
TotalSeq™-A0012 anti-mouse CD117 (c-kit) 2B8 PG
Brilliant Violet 605™ anti-mouse CD117 (c-Kit) 2B8 FC
Alexa Fluor® 700 anti-mouse CD117 (c-Kit) 2B8 FC
TotalSeq™-B0012 anti-mouse CD117 (c-Kit) 2B8 PG
TotalSeq™-C0012 anti-mouse CD117 (c-Kit) 2B8 PG
Spark NIR™ 685 anti-mouse CD117 (c-kit) 2B8 FC
Brilliant Violet 650™ anti-mouse CD117 (c-kit) 2B8 FC
Spark Red™ 718 anti-mouse CD117 (c-kit) (Flexi-Fluor™) 2B8 FC
Spark Blue™ 574 anti-mouse CD117 (c-kit) (Flexi-Fluor™) 2B8 FC
Spark Blue™ 550 anti-mouse CD117 (c-kit) (Flexi-Fluor™) 2B8 FC
Go To Top Version: 2    Revision Date: 06/28/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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