PE anti-mouse Perforin Antibody

Pricing & Availability
Clone
S16009A (See other available formats)
Regulatory Status
RUO
Other Names
Prf1, Pfp, Pfn, Pfn-1
Isotype
Rat IgG2a, κ
S16009A_PE_Perforin_Antibody_1_112217
C57BL/6 splenocytes were stained with CD49b APC. Cells were fixed, permeabilized, and stained with PE anti-mouse perforin (clone S16009A, top) or PE rat IgG2a, κ isotype control (bottom).
  • S16009A_PE_Perforin_Antibody_1_112217
    C57BL/6 splenocytes were stained with CD49b APC. Cells were fixed, permeabilized, and stained with PE anti-mouse perforin (clone S16009A, top) or PE rat IgG2a, κ isotype control (bottom).
  • S16009A_PE_Perforin_Antibody_2_112217
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Cat # Size Price Quantity Check Availability
154305 25 µg $141.00
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154306 100 µg $372.00
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Description

Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse perforin transfected cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone S16009A reacts with perforin from both C57BL/6 and BALB/c strains.

Product Citations
  1. Wang M, et al. 2022. Adv Sci (Weinh). 9:e2202914. PubMed
  2. Pan Y, et al. 2023. Adv Sci (Weinh). 10:e2206792. PubMed
  3. Li H, et al. 2022. Cancer Cell. 40:36. PubMed
  4. Jia JJ, et al. 2022. J Leukoc Biol. 112:157. PubMed
  5. Campisi L, et al. 2022. Nature. 606:945. PubMed
  6. Liu L, et al. 2022. Nat Commun. 13:6740. PubMed
  7. Yang H, et al. 2023. Cell Death Differ. 30:560. PubMed
  8. Xu W 2023. Cell Reports. 42(1):111987. PubMed
  9. Wang W, et al. 2022. World J Gastrointest Oncol. 14:1124. PubMed
  10. Macchi C, et al. 2022. iScience. 25:104435. PubMed
  11. Reyes RM, et al. 2021. Oncoimmunology. 10:2006529. PubMed
  12. Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed
  13. Jiang P, et al. 2021. Nat Methods. 18:1181. PubMed
  14. Goggi JL, et al. 2020. Mol Imaging Biol. 22:1392. PubMed
  15. Wu J, et al. 2020. Immunity. 53:115. PubMed
  16. Chandana M, et al. 2022. Nat Commun. 13:4028. PubMed
  17. Pathania AS, et al. 2022. Mol Ther Oncolytics. 25:308. PubMed
  18. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  19. Lou Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  20. Zhang X, et al. 2021. Nat Commun. 12:4536. PubMed
  21. Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed
  22. Shen H, et al. 2022. Nat Commun. 13:5013. PubMed
RRID
AB_2721638 (BioLegend Cat. No. 154305)
AB_2721638 (BioLegend Cat. No. 154306)

Antigen Details

Structure
70 kD Protein that oligomerizes for pore formation.
Distribution

CTL, NK (Cytoplasmic Granules)

Function
Target cell lysis
Cell Type
NK cells, T cells
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Podack ER, et al. 1988. Immunol. Rev. 103:203.
  2. Konjar S, et al. 2010. Immunology. 131:257.
  3. Trapani JA, et al. 2002. Nat. Rev. Immunol. 2:735.
  4. Osinska I, et al. 2014. Cent. Eur. J. Immnol. 39:109.
Gene ID
18646 View all products for this Gene ID
UniProt
View information about Perforin on UniProt.org
Go To Top Version: 0    Revision Date: 11/29/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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