PE/Cyanine7 anti-human CD235ab Antibody

Pricing & Availability
Clone
HIR2 (See other available formats)
Regulatory Status
RUO
Workshop
VII 70299
Other Names
Glycophorin A/B, GPA/GPB, GYPA, GYPB
Isotype
Mouse IgG2b, κ
HIR2_PECy7_CD235ab_Antibody_090517
Human erythrocytes were first treated with Human TruStain FcX™ then stained with clone HIR2 PE/Cyanine7 (filled histogram) or mouse IgG2b, κ PE/Cyanine7 isotype control (dashed histogram).
  • HIR2_PECy7_CD235ab_Antibody_090517
    Human erythrocytes were first treated with Human TruStain FcX™ then stained with clone HIR2 PE/Cyanine7 (filled histogram) or mouse IgG2b, κ PE/Cyanine7 isotype control (dashed histogram).
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability
306619 25 µg $147.00
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306620 100 µg $323.00
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Description

The HIR2 antibody reacts with a common epitope of glycophorin A (CD235a) and glycophorin B (CD235b). Glycophorin A is the major sialoglycoprotein expressed on red blood cell membrane, and erythroid precursors. Glycophorin A shares strong homology with glycophorin B. The HIR2 antibody recognizes human RBCs and erythroid precursors and is useful in erythroid cell development studies. Mature, non-nucleated red blood cells are characteristically glycophorin A positive, but CD45 and CD71 negative.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.02 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Mason D, et al. Eds. 2002. Leucocyte Typing VII. Oxford University Press. New York.
Product Citations
  1. Worthen CA, et al. 2020. J Invest Dermatol. 140:2515. PubMed
  2. Peslak SA, et al. 2020. Blood Adv. 3.333333333. PubMed
RRID
AB_2716163 (BioLegend Cat. No. 306619)
AB_2716163 (BioLegend Cat. No. 306620)

Antigen Details

Structure
Sialoglycoprotein, 20 kD
Distribution

Erythrocytes

Cell Type
Erythrocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Mason D, et al. Eds. 2002. Leucocyte Typing VII. Oxford University Press. New York.

Gene ID
2993 View all products for this Gene ID
UniProt
View information about CD235ab on UniProt.org
Go To Top Version: 0    Revision Date: 09/05/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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