PE/Cyanine7 anti-human CD25 Antibody

Pricing & Availability
Clone
S20019C (See other available formats)
Regulatory Status
RUO
Other Names
interleukin-2 receptor alpha chain, interluekin-2 receptor, IL2RA, IL-2Rα
Isotype
Mouse IgG1, κ
1_S20019C_PE-Cyanine7_-CD25_FC_062423
PHA-activated human peripheral blood lymphocytes were stained with anti-human CD25 (clone S20019C) PE/Cyanine7 (filled histogram) or mouse IgG1, κ PE/Cyanine7 isotype control (open histogram).
  • 1_S20019C_PE-Cyanine7_-CD25_FC_062423
    PHA-activated human peripheral blood lymphocytes were stained with anti-human CD25 (clone S20019C) PE/Cyanine7 (filled histogram) or mouse IgG1, κ PE/Cyanine7 isotype control (open histogram).
  • 2_S20019C_PE-Cyanine7_-CD25_FC_062423
    Human peripheral blood lymphocytes were stained with anti-human CD25 (clone S20019C) PE/Cyanine7 (left) or mouse IgG1, κ PE/Cyanine7 isotype control (right). Cells were costained with anti-human CD4 APC.
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability
385603 25 tests $145.00
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385604 100 tests $315.00
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Description

CD25 is a 55 kD transmembrane protein also known as interleukin-2 receptor alpha chain. CD25 in combination with CD122 and CD132 form a high affinity IL-2 receptor that binds to IL-2, playing a crucial role in activation of JAK1/JAK3 and STAT5 signaling. It is expressed on activated T-Cells as well as on CD4+ T regulatory cells, some progenitor B-cells and activated monocytes. 

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CD25 transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone S20019C can completely block binding of clone BC96 and clone TÜ69 on target cells and can be blocked by BC96 and by TÜ69.

Clone S20019C does not block the binding of clone S20019D and clone M-A251 on target cells.

Clone S20019C can block IL-2 binding to receptor.

Clone S20019C shows comparable CD25 staining on 4% formaldehyde-fixed cells compared to unfixed.

RRID
AB_3068148 (BioLegend Cat. No. 385603)
AB_3068148 (BioLegend Cat. No. 385604)

Antigen Details

Structure
55 kD transmembrane protein
Distribution

Activated T-Cells, T regulatory Cells, monocytes

Function
IL-2 receptor
Interaction
CD122, CD132
Ligand/Receptor
IL-2
Cell Type
Lymphocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Waldmann T. 1991. J Biol. Chem. 266:2681-4.
  3. Sakaguchi S, et al. 1995. The Journal of Immunology. 155:1151-64.
Gene ID
3559 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org
Go To Top Version: 1    Revision Date: 06/29/2023

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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