PE/Cyanine7 anti-human IL-17A Antibody

Pricing & Availability
Clone
BL168 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin IL-17A, IL-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)
Isotype
Mouse IgG1, κ
BL168_PECyanine7_IL-17A_Antibody_1_081424
PMA + ionomycin-stimulated (6 hours) human peripheral blood mononuclear cells were surface stained with anti-human CD3 (clone UCHT1) FITC. Cells were then fixed, permeabilized, and intracellularly stained with anti-human IL-17A (clone BL168) PE/Cyanine7 (left) or mouse IgG1, κ PE/Cyanine7 isotype control (right). Data shown was gated on the lymphocyte population.
  • BL168_PECyanine7_IL-17A_Antibody_1_081424
    PMA + ionomycin-stimulated (6 hours) human peripheral blood mononuclear cells were surface stained with anti-human CD3 (clone UCHT1) FITC. Cells were then fixed, permeabilized, and intracellularly stained with anti-human IL-17A (clone BL168) PE/Cyanine7 (left) or mouse IgG1, κ PE/Cyanine7 isotype control (right). Data shown was gated on the lymphocyte population.
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability
512315 25 tests $176.00
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Description

IL-17A is the founding member of the IL-17 family, a group of six structurally related pro-inflammatory cytokines. IL-17A, secreted by activated CD4+ Th17 cell subpopulation, elicits multiple biological activities on a variety of cells including: the induction of IL-6, IL-8, G-CSF, and PGE2 production in epithelial, endothelial or fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-κB and costimulation of T cell proliferation. Recent studies demonstrated that, in mice, activated IL-17-secreting CD4+ helper T cells (Th17 cells) mediate an autoimmune arthritis that clinically and immunologically resembles rheumatoid arthritis (RA). Human IL-17A shows 63%, 63%, and 72% amino acid sequence identity to rat IL-17A, mouse IL-17A, and a protein encoded by the ORF13 gene of herpesvirus Saimiri (HVS), respectively.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant full length human IL-17A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. McMurray JL, et al. 2022. Cell Rep. 39:110858. PubMed
  2. Schäbitz A, et al. 2022. Nat Commun. 13:7729. PubMed
  3. Wang S, et al. 2021. Exp Ther Med. 21:37. PubMed
  4. Cohen KW, et al. 2021. Cell Reports Medicine. 2(7):100354. PubMed
  5. Jiang J, et al. 2017. Arthritis Res Ther. 10.1186/s13075-017-1261-9. PubMed
  6. Tchakoute C, et al. 2015. J Infect Dis. 211:338. PubMed
  7. Kim ST, et al. 2021. J Immunother Cancer. 9:. PubMed
  8. Björkander S, et al. 2016. Sci Rep. 6:22083. PubMed
  9. Seenappa LM, et al. 2022. NPJ Vaccines. 7:128. PubMed
  10. Vargas-Inchaustegui D, et al. 2016. J Immunol. 196: 1700 - 1710. PubMed
RRID
AB_2295923 (BioLegend Cat. No. 512315)

Antigen Details

Structure
Cytokine; dimer; ~15 kD (Mammalian)
Bioactivity
Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
Cell Sources
Activated T cell subpopulation
Cell Targets
Fibroblasts, epithelial and endothelial cells, stromal cells
Receptors
IL-17R
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Hirota K, et al. 2007. J. Exp. Med. 204:41.
2. Furuzawa-Carballeda J, et al. 2007. Autoimmun. Rev. 6:169.
3. Witowski J, et al. 2007. Kidney Int. 71:514.
4. Gaffen SL, et al. 2006. Vitam. Horm. 74:255.
5. Hymowitz S, et al. 2001. EMBO J. 20:5332.

Gene ID
3605 View all products for this Gene ID
UniProt
View information about IL-17A on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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