PE/Cyanine7 anti-mouse CD19 Antibody

Pricing & Availability
Clone
6D5 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Rat IgG2a, κ
6D5_PECyanine7_CD19_Antibody_1_071124
C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε (clone 145-2C11) FITC and anti-mouse CD19 (clone 6D5) PE/Cyanine7 (left) or rat IgG2a, κ PE/Cyanine7 isotype control (right).
  • 6D5_PECyanine7_CD19_Antibody_1_071124
    C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε (clone 145-2C11) FITC and anti-mouse CD19 (clone 6D5) PE/Cyanine7 (left) or rat IgG2a, κ PE/Cyanine7 isotype control (right).
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability
115519 25 µg $77.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
115520 100 µg $215.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Application References

(PubMed link indicates BioLegend citation)
  1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
  1. Turecamo SE, et al. 2018. Matrix Biol. 67:01:00. PubMed
  2. Agrafiotis A, et al. 2023. iScience. 26:106055. PubMed
  3. Wang Y, et al. 2023. EBioMedicine. 90:104480. PubMed
  4. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
  5. van Os BW, et al. 2023. Eur Heart J Open. 3:oead013. PubMed
  6. Prior JT, et al. 2023. NPJ Vaccines. 8:80. PubMed
  7. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  8. Zhu Y, et al. 2022. Nat Commun. 13:4282. PubMed
  9. Friesen M, et al. 2022. Brain Pathol. 32:e13032. PubMed
  10. Omer-Javed A, et al. 2022. Cell. 185:2248. PubMed
  11. Millar FR, et al. 2022. Cell Rep. 41:111596. PubMed
  12. Zhang B, et al. 2023. Signal Transduct Target Ther. 8:28. PubMed
  13. Morse M, et al. 2016. Lupus. 25: 81 - 87. PubMed
  14. Barrett N, et al. 2016. Cell Rep. 16: 1039-1054. PubMed
  15. Bussières-Marmen S, et al. 2017. Cell Mol Immunol. 10.1038/cmi.2016.72. PubMed
  16. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  17. Lin YH, et al. 2021. Front Immunol. 12:626418. PubMed
  18. d'Errico P, et al. 2021. Nat Neurosci. Online ahead of print. PubMed
  19. Symeonidou V, et al. 2021. Cell Rep. 37:109900. PubMed
  20. Clark K, et al. 2015. J Autoimmun. Available online 21 August 2015. PubMed
  21. Castañeda-Zegarra S, et al. 2020. Aging (Albany NY). 12:00. PubMed
  22. Yu K, et al. 2021. Cell Reports. 35(12):109273. PubMed
  23. Liao Y, et al. 2017. Virol J. 10.1186/s12985-017-0801-x. PubMed
  24. Yang C, et al. 2020. J Control Release. 326:324. PubMed
  25. Lin CA, et al. 2021. Pharmaceutics. 13:. PubMed
  26. Morgado P, et al. 2014. Infect Immun . 82:4047. PubMed
  27. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  28. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  29. Campana L, et al. 2018. J Immunol. 200:1169. PubMed
  30. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  31. Alberti D, et al. 2021. Curr Protoc. 1:e311. PubMed
  32. Rogawski DS, et al. 2021. Nat Commun. 12:2792. PubMed
  33. Ma M, et al. 2021. Front Immunol. 12:718779. PubMed
  34. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  35. Haugh KA, et al. 2021. eLife. 0.416666666666667. PubMed
  36. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  37. Schaffert A, et al. 2020. Front Immunol. 10:3024. PubMed
  38. Yoshida K, et al. 2022. Sci Rep. 12:17276. PubMed
  39. Wiesner D, et al. 2015. PLoS Pathog. 11:1004701. PubMed
  40. Syed A, et al. 2015. Infect Immun . 83: 3428-3437. PubMed
  41. Calés C, et al. 2016. Mol Cell Biol. 36: 900 - 912. PubMed
  42. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  43. Pereira JA, et al. 2020. Hum Mol Genet. 29:1253. PubMed
  44. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  45. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  46. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  47. Bradley KC, et al. 2019. Cell Rep. 28:245. PubMed
  48. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  49. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  50. Siggs O, et al. 2015. Proc Natl Acad Sci U S A. 112: E5706 - E5714. PubMed
  51. Noviski M, et al. 2018. Elife. 7:e35074. PubMed
  52. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  53. Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed
  54. Challa TD, et al. 2020. Cell Reports. 30(10):3424-3433. PubMed
  55. Wang YH, et al. 2019. Front Immunol. 10:1909. PubMed
  56. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  57. Wu S, et al. 2022. BMC Immunol. 23:17. PubMed
  58. Kang X, et al. 2022. J Immunol Res. 2022:8118577. PubMed
  59. Madenspacher JH, et al. 2020. JCI Insight. 5:00. PubMed
  60. Pillonel V, et al. 2016. Sci Rep. 6:37772. PubMed
  61. Lim S, et al. 2016. PLoS One. 11: 0155689. PubMed
  62. Kim MY, et al. 2018. Cell. 173:1439. PubMed
  63. Matthew B Buechler et al. 2019. Immunity. 51(1):119-130 . PubMed
  64. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  65. Ma K, et al. 2022. iScience. 25:104347. PubMed
  66. Zhang P, et al. 2022. Nat Commun. 13:1588. PubMed
  67. Li M, et al. 2021. Cancers (Basel). 13:. PubMed
  68. Wieghofer P, et al. 2021. EMBO J. 40:e105123. PubMed
  69. Zhou J, et al. 2022. Int J Oncol. 61: . PubMed
  70. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
  71. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
  72. Zhou X, et al. 2021. Cell Reports. 34(1):108601. PubMed
  73. Andersen TK, et al. 2019. NPJ Vaccines. 4:9. PubMed
  74. Ackermann C, et al. 2019. Sci Rep. 9:10624. PubMed
  75. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  76. Onai N, et al. 2020. Int J Mol Sci. :22. PubMed
  77. Vincent B, et al. 2010. J Immunol. 184:4196. PubMed
  78. Ghezraoui H, et al. 2018. Nature. 560:122. PubMed
  79. Willemsen J, et al. 2021. Cell Rep. 37:109977. PubMed
  80. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
  81. Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed
  82. Shimba A et al. 2018. Immunity. 48(2):286-298 . PubMed
  83. Korin B, et al. 2020. Sleep. :43. PubMed
  84. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  85. Zhang Y, et al. 2022. Front Pharmacol. 13:952775. PubMed
  86. Boulch M, et al. 2021. Sci Immunol. 6:. PubMed
  87. Leruste A, et al. 2020. Cancer Cell. 36(6):597-612.e8.. PubMed
  88. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  89. Parigger T, et al. 2021. Int J Mol Sci. 22:. PubMed
  90. Rodrigues KA, et al. 2021. Sci Adv. 7:eabj6538. PubMed
  91. Bansal K, et al. 2017. Nat Immunol. 18:263-273. PubMed
  92. Fusciello M, et al. 2019. Nat Commun. 4.407638889. PubMed
  93. Huang LH et al. 2018. Cell metabolism. 29(2):475-487 . PubMed
  94. Song W, et al. 2022. Immunity. 55:290. PubMed
  95. Zhang B, et al. 2021. Front Neurol. 607370:12. PubMed
  96. Kleiman E, et al. 2016. Proc Natl Acad Sci U S A. 113: E3911 - E3920. PubMed
RRID
AB_313654 (BioLegend Cat. No. 115519)
AB_313654 (BioLegend Cat. No. 115520)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Other Formats

View All CD19 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD19 6D5 FC
Biotin anti-mouse CD19 6D5 FC
FITC anti-mouse CD19 6D5 FC
PE anti-mouse CD19 6D5 FC
PE/Cyanine5 anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Cyanine7 anti-mouse CD19 6D5 FC
Alexa Fluor® 488 anti-mouse CD19 6D5 FC
Alexa Fluor® 647 anti-mouse CD19 6D5 FC,IHC-F
Pacific Blue™ anti-mouse CD19 6D5 FC
Alexa Fluor® 700 anti-mouse CD19 6D5 FC
APC/Cyanine7 anti-mouse CD19 6D5 FC
PerCP anti-mouse CD19 6D5 FC
PerCP/Cyanine5.5 anti-mouse CD19 6D5 FC
Alexa Fluor® 594 anti-mouse CD19 6D5 IHC-F,FC
Brilliant Violet 421™ anti-mouse CD19 6D5 FC,IHC-F
Brilliant Violet 570™ anti-mouse CD19 6D5 FC
Brilliant Violet 605™ anti-mouse CD19 6D5 FC
Brilliant Violet 650™ anti-mouse CD19 6D5 FC
Brilliant Violet 785™ anti-mouse CD19 6D5 FC
Brilliant Violet 510™ anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 (Maxpar® Ready) 6D5 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD19 6D5 FC
Brilliant Violet 711™ anti-mouse CD19 6D5 FC
APC/Fire™ 750 anti-mouse CD19 6D5 FC
TotalSeq™-A0093 anti-mouse CD19 6D5 PG
Brilliant Violet 750™ anti-mouse CD19 6D5 FC
TotalSeq™-B0093 anti-mouse CD19 6D5 PG
Spark Blue™ 550 anti-mouse CD19 6D5 FC
Spark NIR™ 685 anti-mouse CD19 6D5 FC
TotalSeq™-C0093 anti-mouse CD19 6D5 PG
Ultra-LEAF™ Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Fire™ 640 anti-mouse CD19 Antibody 6D5 FC
Spark YG™ 581 anti-mouse CD19 6D5 FC
APC/Fire™ 810 anti-mouse CD19 6D5 FC
Spark YG™ 570 anti-mouse CD19 6D5 IHC-F
Spark Blue™ 574 anti-mouse CD19 Antibody 6D5 FC
Spark Blue™ 515 anti-mouse CD19 6D5 FC
Spark UV™ 387 anti-mouse CD19 6D5 FC
Spark Red™ 718 anti-mouse CD19 (Flexi-Fluor™) 6D5 FC
Go To Top Version: 2    Revision Date: 05/24/2013

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account