PE/Cyanine7 anti-rat CD4 Antibody

Pricing & Availability
Clone
W3/25 (See other available formats)
Regulatory Status
RUO
Other Names
T4, L3T4, W3/25
Isotype
Mouse IgG1, κ
W3slash25_PECy7_021009.jpg
LOU rat splenocytes stained with W3/25 PE/Cyanine7
  • W3slash25_PECy7_021009.jpg
    LOU rat splenocytes stained with W3/25 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability
201515 25 µg $147.00
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201516 100 µg $323.00
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Description

CD4 is a 55 kD glycoprotein also known as T4. Rat CD4 is a member of the immunoglobulin superfamily and is expressed on majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells). CD4 is a T cell co-receptor that interacts with the MHC class II molecule and is involved in T cell activation. 

Technical data sheet

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Rat thymocyte membrane glycoproteins
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The W3/25 antibody has been shown to inhibit IL-2 production by T helper cells and to prevent autoimmune T cell transfer in an MBP induced EAE model in vivo. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1,2, inhibition of IL-2 production3, inhibition of MBP-induced T cell activation in EAE transfer model3.

Application References

(PubMed link indicates BioLegend citation)
  1. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
  2. Shioji K, et al. 2001. Circulation Res. 89:540. (IHC)
  3. Mannie MD, et al. 1993. J. Immunol. 151:7293.
  4. Kurtz CC, et al. 2007. Dev. Comp. Immunol. 31:415. PubMed
Product Citations
  1. Ding X, et al. 2020. Kidney360. 1:106. PubMed
  2. Cho KH, et al. 2022. Front Immunol. 13:930511. PubMed
  3. Beach C, et al. 2023. J Clin Invest. :133. PubMed
  4. Guo Q, et al. 2021. Drug Des Devel Ther. 15:4559. PubMed
RRID
AB_1186087 (BioLegend Cat. No. 201515)
AB_1186087 (BioLegend Cat. No. 201516)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells)

Function
T cell co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Macrophages, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Brideau RJ, et al. 1980. Eur. J. Immunol. 10:609.
2. Clark SJ, et al. 187. P. Natl. Acad. Sci. USA 84:1649.

Gene ID
24936 View all products for this Gene ID 24937 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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