- Clone
- W3/25 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T4, L3T4, W3/25
- Isotype
- Mouse IgG1, κ
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LOU rat splenocytes were stained with CD3 (clone 1F4) PE and CD4 (clone W3/25) PerCP/Cyanine5.5 (left) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 201519 | 25 µg | $124.00 | |||
| 201520 | 100 µg | $317.00 | |||
CD4 is a 55 kD glycoprotein also known as T4. Rat CD4 is a member of the immunoglobulin superfamily and is expressed on majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells). CD4 is a T cell co-receptor that interacts with the MHC class II molecule and is involved in T cell activation.
Product Details
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Rat thymocyte membrane glycoproteins
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. - Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
The W3/25 antibody has been shown to inhibit IL-2 production by T helper cells and to prevent autoimmune T cell transfer in an MBP induced EAE model in vivo. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1,2, inhibition of IL-2 production3, inhibition of MBP-induced T cell activation in EAE transfer model3.
-
Application References
(PubMed link indicates BioLegend citation) -
- Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
- Shioji K, et al. 2001. Circulation Res. 89:540. (IHC)
- Mannie MD, et al. 1993. J. Immunol. 151:7293.
- Kurtz CC, et al. 2007. Dev. Comp. Immunol. 31:415. PubMed
- Product Citations
-
- RRID
-
AB_2814097 (BioLegend Cat. No. 201519)
AB_2814097 (BioLegend Cat. No. 201520)
Antigen Details
- Structure
- Ig superfamily, 55 kD
- Distribution
-
Majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells)
- Function
- T cell co-receptor, T cell activation
- Ligand/Receptor
- MHC class II molecule
- Cell Type
- Macrophages, T cells, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Brideau RJ, et al. 1980. Eur. J. Immunol. 10:609.
2. Clark SJ, et al. 187. P. Natl. Acad. Sci. USA 84:1649. - Gene ID
- 24932 View all products for this Gene ID
- UniProt
- View information about CD4 on UniProt.org
Other Formats
View All CD4 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-rat CD4 | W3/25 | FC,IHC-F |
| FITC anti-rat CD4 | W3/25 | FC |
| PE anti-rat CD4 | W3/25 | FC |
| APC anti-rat CD4 | W3/25 | FC |
| Alexa Fluor® 488 anti-rat CD4 | W3/25 | FC,IHC-F |
| APC/Cyanine7 anti-rat CD4 | W3/25 | FC |
| PE/Cyanine7 anti-rat CD4 | W3/25 | FC |
| PerCP/Cyanine5.5 anti-rat CD4 | W3/25 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-rat CD4
Lou rat splenocytes stained with purified W3/25, followed by... -
FITC anti-rat CD4
LOU rat splenocytes stained with W3/25 FITC -
PE anti-rat CD4
LOU rat splenocytes stained with W3/25 PE -
APC anti-rat CD4
LOU rat splenocytes stained with W3/25 APC -
Alexa Fluor® 488 anti-rat CD4
LOU rat splenocytes stained with W3/25 Alexa Fluor® 488 
Rat frozen spleen section was fixed with 4% paraformaldehyde... -
APC/Cyanine7 anti-rat CD4
LOU rat splenocytes stained with W3/25 APC/Cyanine7 -
PE/Cyanine7 anti-rat CD4
LOU rat splenocytes stained with W3/25 PE/Cyanine7 -
PerCP/Cyanine5.5 anti-rat CD4
LOU rat splenocytes were stained with CD3 (clone 1F4) PE and...