PE/Dazzle™ 594 anti-mouse CD122 (IL-2Rβ) Antibody

Pricing & Availability
Clone
TM-β1 (See other available formats)
Regulatory Status
RUO
Other Names
IL-2 Receptor β chain, IL-2Rβ
Isotype
Rat IgG2b, κ
TM-beta1_PEDZL594_CD122_Antibody_030816
C57BL/6 mouse splenocytes were stained with CD122 (clone TM-β1) PE/Dazzle™ 594 (filled histogram) or rat IgG2b, κ PE/Dazzle™ 594 isotype control (open histogram).
  • TM-beta1_PEDZL594_CD122_Antibody_030816
    C57BL/6 mouse splenocytes were stained with CD122 (clone TM-β1) PE/Dazzle™ 594 (filled histogram) or rat IgG2b, κ PE/Dazzle™ 594 isotype control (open histogram).
Compare all formats See PE/Dazzle™ 594 spectral data
Cat # Size Price Quantity Check Availability
123217 25 µg $159.00
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123218 100 µg $370.00
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Description

CD122 is a 70-75 kD IL-2 receptor β chain also known as IL-2Rβ, which is also shared by the IL-15 receptor. It is constitutively expressed by NK cells and at lower levels by T cells, B cells, monocytes, and macrophages. The IL-2Rβ chain can combine with either the common γ subunit (γc, CD132) alone or with the γc subunit and the IL-2Rα subunit (CD25) to generate intermediate or high affinity IL-2 receptor complexes, respectively. CD122 expression levels can be upregulated by activation. The TM-β1 antibody does inhibit IL-2 binding to the IL-2 receptor. CD122 is expressed on murine, but not human, CD8+ Tregs involved in the maintenance of T cell homeostasis.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Rat T cell line expressing mouse CD122 ( IL-2Rβ)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, blocking of IL-2 binding1, and NK cell depletion2 in vivo. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for blocking of IL-2 binding in vivo and in vitro (Cat. No. 123204).

Application References

(PubMed link indicates BioLegend citation)
  1. Tanaka T, et al. 1991. J. Immunol. 147:2222.
  2. Tanaka T, et al. 1993. J. Exp. Med. 178:1103.
  3. Tanaka T, et al. 1992. Int. Immunol. 4:487.
Product Citations
  1. Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed
  2. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
RRID
AB_2572179 (BioLegend Cat. No. 123217)
AB_2572179 (BioLegend Cat. No. 123218)

Antigen Details

Structure
Ig superfamily, forms high affinity IL-2 receptor with CD25 and CD132 chains or intermediate affinity receptor with CD132 alone, 70-75 kD
Distribution
T and B cells, NK cells, monocytes, macrophages
Function
Critical component of IL-2 and IL-15 signaling
Ligand/Receptor
IL-2, IL-15
Cell Type
B cells, Macrophages, Monocytes, NK cells, T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Minami Y, et al. 1993. Annu. Rev. Immunol. 11:245.
3. Suzuki H, et al. 1995. Science 268:1472.
4. Shi Z, et al. 2009. Eur. J. Immunol. 39:2109.

Gene ID
16185 View all products for this Gene ID
UniProt
View information about CD122 on UniProt.org
Go To Top Version: 1    Revision Date: 03/10/2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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