Ultra-LEAF™ Purified anti-mouse CD122 (IL-2Rβ) Antibody

Pricing & Availability
Clone
TM-β1 (See other available formats)
Regulatory Status
RUO
Other Names
IL-2 Receptor β chain, IL-2Rβ
Isotype
Rat IgG2b, κ
TM-beta1_ULEAF_CD122_Antibody_052819_updated.png
C57BL/6 mouse splenocytes stained with NK-1.1 APC and CD122 (IL-2Rβ) (clone TM-β1) Purified (left) or Purified Rat IgG2b, κ isotype control (right) followed by anti-rat IgG FITC.
  • TM-beta1_ULEAF_CD122_Antibody_052819_updated.png
    C57BL/6 mouse splenocytes stained with NK-1.1 APC and CD122 (IL-2Rβ) (clone TM-β1) Purified (left) or Purified Rat IgG2b, κ isotype control (right) followed by anti-rat IgG FITC.
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Cat # Size Price Quantity Check Availability
123223 100 µg $110.00
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123224 1 mg $149.00
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123225 5 mg $539.00
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123226 25 mg $1408.00
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123227 50 mg $2354.00
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123228 100 mg $3383.00
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CD122 is a 70-75 kD IL-2 receptor β chain also known as IL-2Rβ, which is also shared by the IL-15 receptor. It is constitutively expressed by NK cells and at lower levels by T cells, B cells, monocytes, and macrophages. The IL-2Rβ chain can combine with either the common γ subunit (γc, CD132) alone or with the γc subunit and the IL-2Rα subunit (CD25) to generate intermediate or high affinity IL-2 receptor complexes, respectively. CD122 expression levels can be upregulated by activation. The TM-β1 antibody does inhibit IL-2 binding to the IL-2 receptor. CD122 is expressed on murine, but not human, CD8+ Tregs involved in the maintenance of T cell homeostasis.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Rat T cell line expressing mouse CD122 ( IL-2Rβ)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
IP, Block, Depletion - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, blocking of IL-2 binding1, and NK cell depletion2 in vivo. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for blocking of IL-2 binding in vivo and in vitro (Cat. No. 123204).

Application References

(PubMed link indicates BioLegend citation)
  1. Tanaka T, et al. 1991. J. Immunol. 147:2222.
  2. Tanaka T, et al. 1993. J. Exp. Med. 178:1103.
  3. Tanaka T, et al. 1992. Int. Immunol. 4:487.
Product Citations
  1. Imanishi T, et al. 2020. Cell Reports. 32(3):107911. PubMed
RRID
AB_2810374 (BioLegend Cat. No. 123223)
AB_2810374 (BioLegend Cat. No. 123224)
AB_2810374 (BioLegend Cat. No. 123225)
AB_2810374 (BioLegend Cat. No. 123226)
AB_2810374 (BioLegend Cat. No. 123227)
AB_2810374 (BioLegend Cat. No. 123228)

Antigen Details

Structure
Ig superfamily, forms high affinity IL-2 receptor with CD25 and CD132 chains or intermediate affinity receptor with CD132 alone, 70-75 kD
Distribution
T and B cells, NK cells, monocytes, macrophages
Function
Critical component of IL-2 and IL-15 signaling
Ligand/Receptor
IL-2, IL-15
Cell Type
B cells, Macrophages, Monocytes, NK cells, T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Minami Y, et al. 1993. Annu. Rev. Immunol. 11:245.
3. Suzuki H, et al. 1995. Science 268:1472.
4. Shi Z, et al. 2009. Eur. J. Immunol. 39:2109.

Gene ID
16185 View all products for this Gene ID
UniProt
View information about CD122 on UniProt.org
Go To Top Version: 2    Revision Date: 05/28/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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