- Clone
- NKTA255 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- HCDM listed
- Other Names
- LAIR-1 (Leukocyte-associated Ig-like receptor 1)
- Isotype
- Mouse IgG1, κ
-
Human peripheral blood lymphocytes were stained with anti-human CD4 (clone RPA-T4) FITC and anti-human CD305 (LAIR1) (clone NKTA255) PE/Fire™ 640 (left) or mouse IgG1, κ PE/Fire™ 640 isotype control (right).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 342809 | 25 tests | $176.00 | |||
| 342810 | 100 tests | $382.00 | |||
CD305, also known as LAIR-1 (leukocyte-associated Ig-like receptor-1), is a 40 kD type I transmembrane glycoprotein. LAIR-1 and LAIR-2 are the only members of the LAIR family in the Ig superfamily. LAIR-1 is an inhibitory molecule characterized by ITIMs in the cytoplasmic domain and collagen ligands. CD305 is expressed on NK cells, T cells, B cells, monocytes, dendritic cells, eosinophils, basophils and mast cells. LAIR-1 functions in the inhibition of cell cytotoxicity, cell activation, proliferation and differentiation.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 640 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Fire™ 640 has a maximum excitation of 566 nm and a maximum emission of 639 nm. - Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
-
Application References
(PubMed link indicates BioLegend citation) -
- Poggi A, et al. 1995. Eur. J. Immunol. 25:369
- RRID
-
AB_3106083 (BioLegend Cat. No. 342809)
AB_3106083 (BioLegend Cat. No. 342810)
Antigen Details
- Structure
- 40 kD type I transmembrane protein, cytoplasmic domain contains ITIM motifs, Ig superfamily
- Distribution
-
NK cells, T cell, B cells, monocytes, dendritic cells, eosinophils, basophils and mast cells
- Function
- Inhibition of cell cytotoxicity, cell activation, proliferation and differentiation
- Ligand/Receptor
- collagens
- Cell Type
- B cells, Basophils, Dendritic cells, Eosinophils, Macrophages, Mast cells, Monocytes, NK cells, T cells
- Biology Area
- Immunology, Inhibitory Molecules, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
-
1. Meyaard L, et al. 2008. J. Leukoc. Biol. 83:799
2. Lebbink RJ, et al. 2006. J. Exp. Med. 203:1419 - Gene ID
- 3903 View all products for this Gene ID
- UniProt
- View information about CD305 on UniProt.org
Other Formats
View All CD305 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Alexa Fluor® 647 anti-human CD305 (LAIR1) | NKTA255 | FC |
| PerCP/Cyanine5.5 anti-human CD305 (LAIR1) | NKTA255 | FC |
| TotalSeq™-A0590 anti-human CD305 (LAIR1) | NKTA255 | PG |
| TotalSeq™-C0590 anti-human CD305 (LAIR1) | NKTA255 | PG |
| PE/Fire™ 640 anti-human CD305 (LAIR1) | NKTA255 | FC |
| TotalSeq™-B0590 anti-human CD305 (LAIR1) | NKTA255 | PG |
| FITC anti-human CD305 (LAIR1) | NKTA255 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Alexa Fluor® 647 anti-human CD305 (LAIR1)
CD3+ human peripheral blood lymphocytes stained with NKTA255... -
PerCP/Cyanine5.5 anti-human CD305 (LAIR1)
Human peripheral blood lymphocytes were stained with CD305 (... -
TotalSeq™-A0590 anti-human CD305 (LAIR1)
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TotalSeq™-C0590 anti-human CD305 (LAIR1)
-
PE/Fire™ 640 anti-human CD305 (LAIR1)
Human peripheral blood lymphocytes were stained with anti-hu... -
TotalSeq™-B0590 anti-human CD305 (LAIR1)
-
FITC anti-human CD305 (LAIR1)
Human peripheral blood lymphocytes were stained with anti-hu...