PE/Fire™ 640 anti-human CD66b Antibody

Pricing & Availability
Clone
6/40c (See other available formats)
Regulatory Status
RUO
Other Names
CEACAM8, CD67, CGM6, NCA-95
Isotype
Mouse IgG1, κ
6slash40c_PEFire640_CD66b_Antibody_091120.png
Human peripheral blood granulocytes were stained with anti-human CD66b PE/Fire™ 640 (clone 6/40c) (filled histogram) or mouse IgG1, κ isotype control PE/Fire™ 640 (open histogram).
  • 6slash40c_PEFire640_CD66b_Antibody_091120.png
    Human peripheral blood granulocytes were stained with anti-human CD66b PE/Fire™ 640 (clone 6/40c) (filled histogram) or mouse IgG1, κ isotype control PE/Fire™ 640 (open histogram).
Compare all formats See PE/Fire™ 640 spectral data
Cat # Size Price Quantity Check Availability
392917 25 tests $129.00
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392918 100 tests $352.00
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Description

CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CD66b, expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant soluble human CEACAM8-Fc produced in HEK293 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 640 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Fire™ 640 has a maximum excitation of 566 nm and a maximum emission of 639 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Singer, et al. 2014. PLoS One. 9(4):e94106. PMID: 24743304
  2. Klaile, et al. 2013. Respir Res. 14:85. PMID 23941132
RRID
AB_2876709 (BioLegend Cat. No. 392917)
AB_2876709 (BioLegend Cat. No. 392918)

Antigen Details

Structure
Ig superfamily, CEA antigen group, GPI-linked glycoprotein
Distribution

Granulocytes

Function
Cell adhesion, neutrophil activation
Ligand/Receptor
CD66c
Cell Type
Granulocytes
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Kuijpers T, et al. 1993. J Immunol. 151:4934.
  2. Kuroki M, et al. 1992. J Leuk Biol. 52:551.
Gene ID
1088 View all products for this Gene ID
UniProt
View information about CD66b on UniProt.org
Go To Top Version: 1    Revision Date: 09/11/2020

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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