PerCP/Cyanine5.5 anti-human CD114 (G-CSFR) Antibody

Pricing & Availability
Clone
LMM741 (See other available formats)
Regulatory Status
RUO
Workshop
VI MA98
Other Names
CSF3R, HG-CSFR, Colony Stimulating Factor 3 receptor
Isotype
Mouse IgG1, κ
LMM741_PerCPCyanine55_CD114_Antibody_103118
Human peripheral blood granulocytes were stained with CD114 (G-CSFR, clone LMM741) PerCP/Cyanine5.5 (filled histogram), or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • LMM741_PerCPCyanine55_CD114_Antibody_103118
    Human peripheral blood granulocytes were stained with CD114 (G-CSFR, clone LMM741) PerCP/Cyanine5.5 (filled histogram), or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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346109 25 tests $147.00
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Description

CD114 is the receptor of the colony stimulating factor 3 (CSF3, G-CSF), consist of two 130 kD, type I transmembrane chains that form a homodimer. The extracellular domain consists of an immunoglobulin-like domain, a cytokine receptor homologue domain, and three fibronectin type III repeats. CD114 is expressed in all stages of granulocyte differentiation and in monocytes, platelets, endothelial cells, placenta and trophoblasts. The binding of CSF3, results in the activation of many signaling molecules such as Syk, Lyn, Jak1, Jak2, Tyk2, SOCS3, SOCS1, STAT5, and Shp1, resulting in the expression of different target genes that will increase neutrophil precursor survival, proliferation and maturation. In mature neutrophils, it increases survival, superoxide anion generation, arachidonic acid release, production of myeloperoxidase and leukocyte alkaline phosphatase (LAP).

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CHO cells transfected with hG-CSFR
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Layton JE, et al. 1997. Growth Factors. 14:117
  2. Layton JE, et al. 1999. J. Biol. Chem. 274:17445
  3. Layton JE, et al. 2001. J. Biol. Chem. 276:36779
  4. Gupta K, et al. 2014. Blood. 123:2550. PubMed
Product Citations
  1. Zhang L, et al. 2016. Mol Med Rep. 3092:13. PubMed
RRID
AB_2562418 (BioLegend Cat. No. 346109)

Antigen Details

Structure
Single chain type I transmembrane molecule of 130 kD. The extracellular domain consists of an immunoglobulin-like domain, a cytokine receptor homologue domain, and three fibronectin type III repeats. To function as a receptor, this molecules form a homod
Distribution

Granulocytes (in all stages of differentiation), monocytes, platelets, endothelial cells, placenta and trophoblasts.

Function
Stimulates survival, proliferation, and maturation of neutrophil precursors. In mature neutrophils increases survival, superoxide anion generation, arachidonic acid release, production of myeloperoxidase and leukocyte alkaline phosphatase (LAP).
Interaction
Syk, Lyn, Jak1, Jak2, Tyk2, SOCS3, SOCS1, STAT5, Shp1.
Ligand/Receptor
CSF3(G-CSF)
Bioactivity
Stimulate the proliferation and differentiation of neutrophils precursors and the activation of mature neutrophils.
Cell Type
Endothelial cells, Granulocytes, Monocytes, Neutrophils, Platelets
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Starnes LM, et al. 2009. Blood 114:1753
2. Skokowa J, et al. 2009. Nat Med. 15:151
3. Ai J, et al. 2008. PLoS One. 3:e3422
4. Germeshausen M, et al. 2008. Curr Opin Hematol. 15:332
5. Marino VJ, Roguin LP, et al. 2008. J Cell Biochem. 103:1512
6. Irandoust MI, et al. 2007. EMBO J. 2:1782

Gene ID
1441 View all products for this Gene ID
UniProt
View information about CD114 on UniProt.org
Go To Top Version: 2    Revision Date: 10/31/2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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