PerCP/Cyanine5.5 anti-human CD3 Antibody

Pricing & Availability
Clone
OKT3 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
T3, CD3ε
Isotype
Mouse IgG2a, κ
OKT3_PerCPCy5.5_CD3_Antibody_FC_100312
Human peripheral blood lymphocytes were stained with CD3 (clone OKT3) PerCP/Cyanine5.5 (filled histogram) or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • OKT3_PerCPCy5.5_CD3_Antibody_FC_100312
    Human peripheral blood lymphocytes were stained with CD3 (clone OKT3) PerCP/Cyanine5.5 (filled histogram) or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability
317335 25 tests $112.00
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317336 100 tests $229.00
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Description

CD3ε is a 20 kD chain of the CD3/T cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex.

Clone OKT3 can block the binding of clones SK7 and UCHT1.4 The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
  4. Li B, et al. 2005. Immunology 116:487.
  5. Jeong HY, et al. 2008. J. Leuckocyte Biol. 83:755. PubMed
  6. Alter G, et al. 2008. J. Virol. 82:9668. PubMed
  7. Manevich-Mendelson E, et al. 2009. Blood 114:2344. PubMed
  8. Pinto JP, et al. 2010. Immunology. 130:217. PubMed
  9. Biggs MJ, et al. 2011. J. R. Soc. Interface. 8:1462. PubMed
Product Citations
  1. Aschmoneit N, et al. 2021. J Immunother Cancer. 9:. PubMed
  2. Eiva MA, et al. 2022. Eur J Immunol. 52:96. PubMed
  3. Barnes CO, et al. 2022. Sci Adv. 8:eabp8155. PubMed
  4. Khuisangeam N, et al. 2022. J Immunol Res. 2022:2449373. PubMed
  5. Beck C, et al. 2023. Biomedicines. 11:. PubMed
  6. Kaku CI, et al. 2023. Nat Commun. 14:2751. PubMed
  7. Lee E, et al. 2021. Sci Rep. 11:14841. PubMed
  8. Kasper M, et al. 2021. Elife. 10:. PubMed
  9. Sim J, et al. 2019. MAbs. 11:1036. PubMed
  10. Wang J, et al. 2020. Cell. 183:1264. PubMed
  11. Zhou Y, et al. 2017. Front Cell Infect Microbiol. 7:457. PubMed
  12. Billing F, et al. 2021. ACS Appl Mater Interfaces. 13:55534. PubMed
  13. Narsale A, Moya R, Robertson H 2016. Data Brief. 8: 1348-51. PubMed
  14. Tian X, et al. 2021. Annals of Translational Medicine. 9(9):781. PubMed
  15. Zhang Z, et al. 2021. Acta Pharm Sin B. 11:1965. PubMed
  16. Bulgarelli J, et al. 2021. Front Immunol. 12:778459. PubMed
  17. Aschmoneit N, et al. 2022. Oncoimmunology. 11:2028961. PubMed
  18. Riquelme SA, et al. 2020. Cell Metabolism. 31(6):1091-1106.e6. PubMed
  19. Wu X, et al. 2021. Front Immunol. 12:731767. PubMed
  20. Aschmoneit N, et al. 2021. Sci Rep. 11:13880. PubMed
  21. Mudd PA, et al. 2022. Cell. 185:603. PubMed
  22. Alonso R, et al. 2022. Blood Adv. :. PubMed
  23. Schoofs T et al. 2019. Immunity. 50(6):1513-1529 . PubMed
  24. Hakki S, et al. 2022. Sci Rep. 12:1427. PubMed
  25. Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed
  26. Collinson–Pautz MR, et al. 2019. Leukemia. 33:2195. PubMed
  27. Albayati Z, et al. 2017. Scand J Immunol. 86:396. PubMed
  28. Ott M, et al. 2020. JCI Insight. 5(17):134386. PubMed
RRID
AB_2561627 (BioLegend Cat. No. 317335)
AB_2561627 (BioLegend Cat. No. 317336)

Antigen Details

Structure
Ig superfamily, the subunits CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) form the CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
  2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
  3. Lanier L, et al. 1986. J. Immunol. 137:2501.
Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org
Go To Top Version: 3    Revision Date: 05/24/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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