PerCP/Cyanine5.5 anti-mouse CD170 (Siglec-F) Antibody

Pricing & Availability
Clone
S17007L (See other available formats)
Regulatory Status
RUO
Other Names
Siglec-5
Isotype
Rat IgG2a, κ
S17007L_PerCPCyanine5_CD170_Antibody_020521
C57BL/6 mouse splenocytes were stained with anti-mouse CD193 (CCR3) (clone J073E5) FITC and anti-mouse CD170 (Siglec-F) (clone S17007L) PerCP/Cyanine5.5 (left) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
  • S17007L_PerCPCyanine5_CD170_Antibody_020521
    C57BL/6 mouse splenocytes were stained with anti-mouse CD193 (CCR3) (clone J073E5) FITC and anti-mouse CD170 (Siglec-F) (clone S17007L) PerCP/Cyanine5.5 (left) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability
155525 25 µg $159.00
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155526 100 µg $375.00
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Description

CD170, also known as Siglec-F, Siglec-5, is a member of the Sialic acid-binding Ig-like lectin family, type I single pass transmembrane protein, with 4 extracellular Ig-like domains and 2 ITIM motifs in the cytoplasmic domain; preferentially binds [alpha]-2,3-linked sialic acid. Siglec F is expressed in eosinophils, alveolar macrophages and intestinal microfold (M) cells and induces apoptosis of the lung eosinophis during allergic asthma.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. 

Excitation Laser
Blue Laser (488 nm)
RRID
AB_2890714 (BioLegend Cat. No. 155525)
AB_2890714 (BioLegend Cat. No. 155526)

Antigen Details

Structure
Member of the Sialic acid-binding Ig-like lectin family, type I, single pass, transmembrane protein, 4 extracellular Ig-like domains, 2 ITIM motifs in the cytoplasmic domain
Distribution

Eosinophils, alveolar macrophages, intestinal microfold (M) cells

Function
Induces apoptosis in eosinophils
Ligand/Receptor
[alpha]-2,3-linked sialic acid
Cell Type
Eosinophils, Macrophages
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity
Molecular Family
CD Molecules, Siglec Molecules
Antigen References
  1. Gicheva N, et al. 2016. Biochem. Biophys. Res. Commun. 479:1.
  2. Kiwamoto T, et al. 2015. J. Allergy Clin. Immunol. 135:1329.
  3. Suzukawa M, et al. 2013. J. Immunol. 190:5939.
  4. Patnode ML, et al. 2013. J. Biol. Chem. 288:26533.
Gene ID
233186 View all products for this Gene ID
UniProt
View information about CD170 on UniProt.org
Go To Top Version: 1    Revision Date: 02/05/2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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