PerCP/Cyanine5.5 anti-mouse CD45RB Antibody

Pricing & Availability
Clone
C363-16A (See other available formats)
Regulatory Status
RUO
Other Names
CD45RB
Isotype
Rat IgG2a, κ
C363-16a_PerCPCy55_111709
C57BL/6 mouse splenocytes stained with C363-16A PerCP/Cyanine5.5
  • C363-16a_PerCPCy55_111709
    C57BL/6 mouse splenocytes stained with C363-16A PerCP/Cyanine5.5
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability
103313 25 µg $141.00
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103314 100 µg $347.00
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Description

CD45RB is an isoform of CD45 with exon 5 splicing (encodes B determinant). It is a 220 kD glycoprotein expressed on peripheral B cells, naïve T cells, thymocytes, weakly on macrophages, and dendritic cells. It plays a critical role in TCR and BCR signaling. As T cells become activated and progress from naïve to memory cells, CD45RB expression is downregulated. Additionally, functionally distinct CD4+ T cell subsets, which secrete differing cytokine profiles, can be separated by CD45RB intensity. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4 and Thy-1.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Cloned mouse Th2 cell lines
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Bottomly K, et al. 1989. Eur. J. Immunol. 19:617.
  2. Norian LA and Allen PM. 2004. J. Immunol. 173:835.
Product Citations
  1. Giampaolo S, et al. 2023. iScience. 26:106234. PubMed
  2. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
RRID
AB_1953290 (BioLegend Cat. No. 103313)
AB_1953290 (BioLegend Cat. No. 103314)

Antigen Details

Structure
Phosphatase receptor (CD45 gene exon 5), 220 kD
Distribution

Peripheral B cells, naïve T cells, most thymocytes, low density on macrophages and dendritic cells

Function
Phosphatase, T cell development and activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, Thy1
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Thymocytes, Tregs
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45RB on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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