PerCP/Cyanine5.5 anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody

Pricing & Availability
Clone
RB6-8C5 (See other available formats)
Regulatory Status
RUO
Other Names
Gr-1
Isotype
Rat IgG2b, κ
RB6-8C5_PerCPCyanine55_Ly-6G-Ly-6C_Antibody_101818
C57BL/6 mouse bone marrow were stained with CD11b FITC and Ly-6G/Ly-6C (clone RB6-8C5) PerCP/Cyanine5.5 (left), or Rat IgG2b, κ PerCP/Cyanine5.5 isotype control (right). Data shown was gated on the myeloid cell population.
  • RB6-8C5_PerCPCyanine55_Ly-6G-Ly-6C_Antibody_101818
    C57BL/6 mouse bone marrow were stained with CD11b FITC and Ly-6G/Ly-6C (clone RB6-8C5) PerCP/Cyanine5.5 (left), or Rat IgG2b, κ PerCP/Cyanine5.5 isotype control (right). Data shown was gated on the myeloid cell population.
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability
108427 25 µg $103.00
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108428 100 µg $211.00
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Description

Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. In bone marrow, the expression levels of Gr-1 directly correlate with granulocyte differentiation and maturation; Gr-1 is also transiently expressed on bone marrow cells in the monocyte lineage. Immature Myeloid Gr-1+ cells play a role in the development of antitumor immunity.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Raised against granulocytes of mouse origin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C19. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A819. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.420.

The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity2, in vivo depletion3-5,9, immunoprecipitation1, immunohistochemical staining6 (including paraffin-embedded sections9,16,33-35, acetone-fixed frozen sections11 and zinc-fixed sections15), and Western blotting7. RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs)20.

Special Note: For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 108436).

Application References

(PubMed link indicates BioLegend citation)
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (IP)
  2. Brummer E, et al. 1984. J. Leukocyte Biol. 36:505. (CMCD)
  3. Stoppacciaro A, et al. 1993. J. Exp. Med. 178:151. (Deplete)
  4. Tumpey TM, et al. 1996. J. Virol. 70:898. (Deplete)
  5. Czuprynski CJ, et al. 1994. J. Immunol. 152:1836. (Deplete)
  6. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
  7. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (WB)
  8. Engwerda CR, et al. 2004. Am. J. Pathol. 165:2123.
  9. Brown CR, et al. 2004. Infect. Immun. 72:4956. (Deplete, IHC)
  10. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC) PubMed
  11. Li M, et al. 2006. P. Natl. Acad. Sci USA 103:11736. (IHC)
  12. Dzhagalov I, et al. 2007. Blood 109:1620. (FC) PubMed
  13. Fazilleau N, et al. 2007. Nature Immunol. 8:753. (FC) PubMed
  14. Heuser M, et al. 2007. Blood 110:1639. (FC) PubMed
  15. Wang T, et al. 2007. Infect. Immun. 75:1144. (IHC)
  16. Bosio CM, et al. 2007. J. Immunol. 178:4538. (IHC)
  17. Boehme SA, et al. 2009. Int. Immunol. 21:81. (IHC)
  18. Piao Y, et al. 2012. Neuro Oncol. 14:1379. PubMed
  19. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  20. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  21. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
  22. Fan Q, et al. 2014. Cancer Res. 74:471. PubMed
  23. Korrer MJ, et al. 2014. PLoS One. 9:91370. PubMed
  24. Morshed M, et al. 2014. J Immunol. 192:5314. PubMed
  25. Collins C, et al. 2014. PNAS. 111:9899. PubMed
  26. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
  27. Bianchi G, et al. 2014. Cell Death Dis. 5:1135. PubMed
  28. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  29. Roderick JE, et al. 2014. PNAS. 111:14436. PubMed
  30. Distel E, et al. 2014. Circ Res. 115:759. PubMed
  31. Iwai H, et al. 2015. Tuberculosis. 95:246. PubMed
  32. Charmsaz S, et al. 2015. PLoS One. 10:130692. PubMed
  33. Whiteland J, et al. 1994 J Histochem Cytochem 43:3 (IHC-P)
  34. Brown C, et al. 2003 J Immunology 171:2 (IHC-P)
  35. Obregon-Henao A, et al. PLoS One 8:11 (IHC-P)
Product Citations
  1. Wang X, et al. 2019. Cell Res. 29:787. PubMed
  2. Lang J, et al. 2020. Nat Commun. 11:1338. PubMed
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  5. Meyers AK, et al. 2023. Cell Rep. 42:111941. PubMed
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  7. Däbritz J, et al. 2016. Sci Rep. 6:20584. PubMed
  8. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  9. Tripathi H, et al. 2020. J Mol Cell Cardiol. 149:95. PubMed
  10. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  11. Kim JS, et al. 2020. Immunity. 54(1):176-190.e7. PubMed
  12. Giambra V et al. 2018. Cell stem cell. 23(5):714-726 . PubMed
  13. Lee H, et al. 2020. Cell Metabolism. 31(4):822-836. PubMed
  14. Liang S, et al. 2022. Acta Pharm Sin B. 12:2494. PubMed
  15. Lutzmann M, et al. 2019. Cell Rep. 28:2851. PubMed
  16. Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed
  17. Liang Q, et al. 2022. iScience. 25:104043. PubMed
  18. Wang H, et al. 2019. Immunotherapy. 11:101. PubMed
  19. Wang X, et al. 2020. Signal Transduct Target Ther. 5:35. PubMed
  20. Kobayashi H, et al. 2020. STAR Protoc. 1:100078. PubMed
  21. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  22. Xu X, et al. 2021. J Nanobiotechnology. 19:376. PubMed
  23. Lebratti T, et al. 2021. eLife. 10:00. PubMed
  24. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
  25. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  26. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  27. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  28. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  29. Ajina R, et al. 2021. Cancer Immunol Res. 9:386. PubMed
  30. Chen L, et al. 2021. Cell Death Differ. 28:1880. PubMed
  31. Hönes J, et al. 2017. Sci Rep. . 10.1038/s41598-017-15866-9. PubMed
  32. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  33. Li L, et al. 2015. J Am Soc Nephrol. 26: 2183-2197. PubMed
  34. Qi S et al. 2016. eLife. 5 pii: e14756. PubMed
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  36. Bugler-Lamb AR, et al. 2021. Mol Metab. 49:101197. PubMed
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  38. Tripathi H, et al. 2020. Stem Cell Rev Rep. 0.953472222. PubMed
  39. Jamali A, et al. 2020. Cell Reports. 32(9):108099. PubMed
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  42. Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed
  43. Zhang P, et al. 2022. Nat Commun. 13:1588. PubMed
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  48. Li L, et al. 2014. J Immunol. 192:1034. PubMed
  49. Richardson ET, et al. 2015. PLoS One. 10: 1371. PubMed
  50. Wang Z, et al. 2020. J Cell Mol Med. . PubMed
  51. Dallari S, et al. 2021. Cell Host Microbe. 29(6):1014-1029.e8. PubMed
  52. Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed
  53. Deliyannis G, et al. 2021. JCI Insight. 6:. PubMed
  54. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
  55. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  56. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  57. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  58. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
  59. Zhang X, et al. 2022. J Exp Clin Cancer Res. 41:246. PubMed
  60. Matsushita N, et al. 2016. Sci Rep. 6:31266. PubMed
  61. Petriello MC, et al. 2018. Toxicol Sci. 162:548. PubMed
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RRID
AB_893558 (BioLegend Cat. No. 108427)
AB_893558 (BioLegend Cat. No. 108428)

Antigen Details

Structure
21-25 kD
Distribution

Granulocytes, monocytes

Cell Type
Granulocytes, Monocytes, Neutrophils
Biology Area
Immunology, Innate Immunity
Antigen References

1. Fleming TJ, et al. 1993. J. Immunol. 151:2399.
2. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
3. Goni O, et al. 2002. Int. Immunol. 14:1125.

Gene ID
17067 View all products for this Gene ID 546644 View all products for this Gene ID
UniProt
View information about Ly-6G Ly-6C on UniProt.org

Other Formats

View All Ly-6G/Ly-6C Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Biotin anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC,IHC,IP,WB
FITC anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
PE anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
PE/Cyanine5 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Purified anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC,IHC,IP,WB
PE/Cyanine7 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Alexa Fluor® 488 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC,IHC
Alexa Fluor® 647 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC,SB
Alexa Fluor® 700 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Brilliant Violet 711™ anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
APC/Cyanine7 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Pacific Blue™ anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
PerCP/Cyanine5.5 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
PerCP anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Brilliant Violet 421™ anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Brilliant Violet 570™ anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Ultra-LEAF™ Purified anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC,IP,CMCD,Depletion,IHC,WB
Brilliant Violet 510™ anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Brilliant Violet 605™ anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Brilliant Violet 650™ anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Alexa Fluor® 594 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 IHC-F,FC
Purified anti-mouse Ly-6G/Ly-6C (Gr-1) (Maxpar® Ready) RB6-8C5 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
APC/Fire™ 750 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
TotalSeq™-A0116 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 PG
TotalSeq™-C0116 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 PG
TotalSeq™-B0116 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 PG
Spark Blue™ 550 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
APC/Fire™ 810 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Spark Violet™ 423 anti-mouse Ly-6G/Ly-6C (GR-1) Antibody RB6-8C5 FC
Spark UV™ 387 anti-mouse Ly-6G/Ly-6C (GR-1) RB6-8C5 FC
Spark Violet™ 538 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Spark PLUS UV395™ anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Spark Red™ 718 anti-mouse Ly-6G/Ly-6C (Gr-1) (Flexi-Fluor™) RB6-8C5 FC
Spark Blue™ 574 anti-mouse Ly-6G/Ly-6C (Gr-1) (Flexi-Fluor™) RB6-8C5 FC
Go To Top Version: 3    Revision Date: 10/18/2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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