Purified anti-ATG5 Antibody

Pricing & Availability
Clone
177.19 (See other available formats)
Regulatory Status
RUO
Other Names
Autophagy protein 5
Isotype
Mouse IgG1, κ
1_177dot19_PURE_ATG5_Antibody_WB_082917
Western blot of anti-ATG5 antibody (clone 177.19). The blot was incubated with the primary antibody at 0.2 µg/ml overnight at 4°C, followed by the incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody (cat# 405306). Enhanced chemiluminescence was used as the detection system. Lysates: 10 µg. Recombinant human ATG5: 5 ng.
  • 1_177dot19_PURE_ATG5_Antibody_WB_082917
    Western blot of anti-ATG5 antibody (clone 177.19). The blot was incubated with the primary antibody at 0.2 µg/ml overnight at 4°C, followed by the incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody (cat# 405306). Enhanced chemiluminescence was used as the detection system. Lysates: 10 µg. Recombinant human ATG5: 5 ng.
  • 2_177dot19_PURE_ATG5_Antibody_ICC_021518
    ICC staining of anti-ATG5 antibody (clone 177.19) on Hela cells. The cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and blocked with 2% normal goat serum and 0.02%BSA. The cells were then stained with 0.5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-Mouse IgG (Cat. No. 405326) for one hour at room temperature. Cells were counterstained with Phalloidin™ Green 488 (Cat. No. 424201) and DAPI to visualize actin filaments and nuclei, respectively. The images were captured with a 60X objective. Scale bar: 50 µm.
  • 3_177point19_Purified_ATG5_Antibody_2_IHCP_080516
    IHC staining of anti-ATG5 antibody (clone 177.19) on formalin-fixed paraffin-embedded normal human brain tissue. Following antigen retrieval using sodium citrate, the tissue was incubated with the primary antibody for 60 minutes at room temperature at 10 µg/ml. BioLegend's Ultra-Streptavidin (USA) HRP Detection Kit was used for detection.
  • 4_177dot19_PURE_ATG5_Antibody_4_WB_082917
    Western blot of purified anti-ATG5 antibody (clone 177.19). Lane 1: Molecular weight marker; Lane 2: 20 µg of Drosophila head lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 436302). Enhanced chemiluminescence was used as the detection system.
  • 5_177dot19_PURE_ATG5_Antibody_4_ICC_010620
    ICC staining of purified anti-ATG5 antibody (clone 177.19) on Drosophila S2 cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation wit 2.5 µg/mL of Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 60X objective. Scale bar: 20 µm
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847401 25 µg $112.00
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847402 100 µg $288.00
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Description

Autophagy protein 5 (ATG5) is involved in autophagic vesicle formation. ATG5 forms a complex with autophagy protein 12 (ATG12), which has E3 ubiquitin ligase activity, and is associated with autophagosome vesicle formation. ATG5 detaches from membranes immediately before or after autophagosome formation. ATG5 is also involved in mitochondrial quality control after oxidative damage, the innate anti-viral immune response, and apoptosis.

Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat, Drosophila
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
ATG5 peptide conjugated to KLH.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IHC-P - Verified
Direct ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL in human, mouse, and rat and 1.0 - 10 µg/mL in Drosophila For immunocytochemistry, a concentration range of 0.1 - 1.0 μg/mL is recommended in human, mouse, rat and 1.0 - 5.0 μg/mL in Drosophila. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 2.0 - 10 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Reactivity to Drosophila was only verified with the purified format.

RRID
AB_2734618 (BioLegend Cat. No. 847401)
AB_2734618 (BioLegend Cat. No. 847402)

Antigen Details

Structure
Human ATG5 is a 275 amino acid protein with a molecular mass of 32 kD.
Distribution

Tissue distribution: Ubiquitous.
Cellular distribution: Vacuole, lysosome, and to a lesser extent mitochondria.

Function
ATG5 is involved in many cellular processes including autophagic vesicle formation, mitochondrial quality control after oxidative damage, innate anti-viral immune response, and apoptosis.
Interaction
ATG12, LC3B, ATG16, TECPR1, Autophagy-linked FYVE protein.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance
Molecular Family
Autophagosome Markers
Antigen References

1. Uchiyama Y, et al. 2008. Histochem. Cell Biol. 129:407. PubMed
2. Wu H, et al. 2014. Int. J. Biol. Sci. 10:1072. PubMed
3. Subramani S, Malhotra V. 2013. EMBO Rep. 14(2):143. PubMed

Gene ID
9474 View all products for this Gene ID
UniProt
View information about ATG5 on UniProt.org
Go To Top Version: 2    Revision Date: 08/16/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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